In this paper, the project of an air conditioning system for a vaccine production center is presented. The building is located in the city of Zagreb, and all the relevant climate data necessary for calculations are defined for the meteorological station Maksimir. The project includes the thermodynamic calculation of the design heat load according to the HRN EN 12831-1:2017 standard and the design cooling load according to the VDI 2078:2015 standard. Additionally, the project involves an hourly calculation of heating and cooling needs for the first floor of the building using the dynamic hourly method according to the HRN EN ISO 13790 standard. It also includes an hourly calculation according to the national Algorithm for the calculation of energy needs for the application of the air conditioning systems for heating and cooling of buildings. The laboratory for vaccine production is categorized as a clean room, so the air conditioning system in this case will exclusively be an air system. The supply and exhaust of air will be done through a centralized air conditioning system with an air conditioning chamber located on the top of the building, using distribution supply and return ductings. The available heating medium for the system will be hot water in a 70/55 °C regime, while the cooling medium will be cold water with a 30% ethylene-glycol solution in a 7/12 °C regime. The energy calculation gives the monthly amount of thermal energy needs for the elements of the air conditioning unit of system 8 and 10, which are: heater, cooler, steam humidifier and reheater. It has been shown that there is a significant difference in the energy needs for thermal energy between these two systems, where the total needs for system 10 are significantly higher due to the year-round maintenance of air humidity in the vaccine production zone.