Title HISTOLOŠKE TEHNIKE ZA PRIKAZ ŽIVČANOG TKIVA
Title (english) HISTOLOGICAL TECHNIQUES OF NERVE TISSUE VISUALIZATION
Author Ilda Pozderac
Mentor Ester Pernjak-Pugel (mentor)
Committee member Dijana Detel (predsjednik povjerenstva)
Committee member Lara Batičić Pučar (član povjerenstva)
Committee member Ester Pernjak-Pugel (član povjerenstva)
Granter University of Rijeka Faculty of Health Studies (Department of Laboratory and Radiological Diagnostics) Rijeka
Defense date and country 2018-07-03, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Cytology, Histology and Embryology
Abstract Cilj ovog istraživanja je vizualizacija histološke građe živčanog tkiva pomoću različitih tehnika bojenja, kao i otkrivanje pogodnosti ovih tehnika za diferenciranje pojedinih komponenti koje nalazimo u živčanom tkivu.
Živčano tkivo nalazimo u građi središnjeg i perifernog živčanog sustava. Središnji živčani sustav čine mozak i kralježnična moždina, dok se periferni živčani sustav sastoji od živčanih vlakana koji formiraju živce te od malih nakupina perikariona živčanih stanica koji izgrađuju
... More ganglija. Živčani sustav je odgovoran za održavanje unutarnje sredine organizma unutar normalnih vrijednosti, kao i za osiguravanje i usklađivanje svih oblika interakcije s okolinom.
Živčano tkivo možemo pokazati koristeći različite histološke i imunohistokemijske tehnike bojenja, koje se odabiru u ovisnosti od područja interesa. U istraživanju su se koristila mišja tkiva, dobivena iz životinja starosti 3 tjedna, koja su nakon fiksacije paraformaldehidom prošla parafinski postupak dobivanja histoloških preparata. Kao rezultat, hemalaun-eozin (HE) bojenje je dalo uvid u osnovnu strukturu histološkog preparata. Cresyl-violet bojenje je specifična i jedna od najviše korištenih tehnika za prikaz živčanog tkiva koja omogućava dobro diferenciranje oblika i smještaja perikariona te prikaz izgleda jezgre i jezgrice. Kluver-Barrera bojenje se koristilo zbog afiniteta boje za mijelinske ovojnice. Pored ovih tehnika bojenja, koristile su se i različite imunokemijske metode u kojima se uz pomoć specifičnih protutijela prikazuju različite strukture na preparatima. Za imunohistokemijski prikaz premitotičkih neurona korišten je rani biomarker živčanih stanica - class III beta tubulin, a pored njega i neuN protutijelo koje se veže za neuronalni nuklearni antigen. Za bojenje Purkinjeovih stanica korišteno je specifično protutijelo Calbindin-D28k koje omogućava dobar prikaz oblika ovih stanica, dok se za prikaz radijalnih glija stanica koristilo anti-GFAP protutijelo. Less
Abstract (english) The aim of the study was to visualize the histological structure of the nerve tissue using different staining methods, as well as to discover suitable techniques for differentiation of tissue components that can be found in the nerve tissue.
Nerve tissue is anatomicaly divided into central and peripheral nervous system. Central nervous system consists of the brain and spinal cord, while the peripheral nervous system contains branching peripheral nerves and ganglia. Nervous system is
... More responsible for keeping the inner environment of the organism in normal values and to ensure and harmonize all sorts of communication of the organism with its surroundings.
Nerve tissue can be shown using different histological and immunohistochemical staining methods that are chosen based on the area of interest. In this study we used the nerve tissue of a laboratory mouse (3 weeks old) that went through the paraffin processing. As a result, hemalaun-eosin (HE) staining gave a good insight into the basic structure of the tissue itself. Cresyl-violet solution is specific, and it is one of the most used techniques for nerve tissue visualization because with its use the difference between shapes and positions of pericarions can be well seen, as well as the appearance of nucleus and nucleolus. Kluver-Barrera staining is used because of its affinity for the myelin sheath. Besides these aforementioned methods, some immunohistochemical techniques were used in the study. In these methods different structures of the tissue were visualized by using specific antibodies. Early biomarker of nerve cells called the class III beta tubulin was used for the immunohistochemical review of the pre-mitotic neurons. Besides this antibody, neuN antibody was used because it bonds to the neural nuclear antigen. Specific antibody Calbindin-D28k was used for staining of the Purkinje cells, because it provides a good insight into the shape of these cells, while anti-GFAP antibody was used for radial glial cells. Less
Keywords
histološki preparat
histokemija
imunohistologija
laboratorijske životinje
parafinski postupak
živčano tkivo
Keywords (english)
histochemistry
histological section
immunohistochemistry
laboratory animals
nerve tissue
paraffin procedure
Language croatian
URN:NBN urn:nbn:hr:184:518522
Study programme Title: Professional study of Medical and Laboratory Diagnostics (Biomedicine and Healthcare; clinical medical sciences) Study programme type: professional Study level: undergraduate Academic / professional title: stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike (stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resource Text
File origin Born digital
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Created on 2018-11-20 13:57:24