Title BAKTERIJSKI BIOFILM NA KROMIRANIM PERLATORIMA
Title (english) BACTERIAL BIOFILM ON CHROMIUM PERLATORS
Author Tamara Dokmanović
Mentor Maja Abram (mentor)
Mentor Ivana Gobin (mentor)
Committee member Marina Bubonja Šonje (predsjednik povjerenstva)
Committee member Mateja Ožanič (član povjerenstva)
Committee member Maja Abram (član povjerenstva)
Granter University of Rijeka Faculty of Health Studies (Department of Laboratory and Radiological Diagnostics) Rijeka
Defense date and country 2018-07-13, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Medical Microbiology
Abstract Voda u bolničkom distribucijskom sustavu može biti rezervoar potencijalno patogenih mikroorganizama koji mogu dovesti do razvoja infekcija povezanih sa zdravstvenom skrbi. Stoga su mikrobiološki analizirani uzorci vodovodne vode te kromirani perlatori s 15 slavina uzorkovanih u jedinicama intenzivnog liječenja KBC Rijeka.
Od ukupnog broja obrađenih uzoraka vode, zbog prevelikog UBK, četiri uzorka (27%) bila su mikrobiološki neprihvatljiva. Postupkom membranske filtracije iz svih je uzoraka vode isključena prisutnost različitih bakterijskih vrsta (S. aureus, B. cepacia, L. pneumophila, Aeromonas spp.), no u dva je dokazan P. aeruginosa (>103/100 mL). Uzorkovani perlatori su obrađeni u ultrazvučnoj kupelji kako bi se odstranio biofilm s površine i povećala osjetljivost mikrobiološke obrade. Samo na dva od 14 analiziranih perlatora nije bio prisutan bakterijski biofilm, dok je s preostalih 12 perlatora izolirano 19 različitih vrsta bakterija. Trinaest bakterija izoliranih iz biofilma s perlatora pripadao je različitim sojevima Pseudomonas aeruginosa, tri vrsti Stenotrophomonas maltophilia,te po jedan izolat vrstama Sphingomonas paucimobilis, Alcaligenes faecalis i Enterobacter cloacae. Sojevi izolirani iz biofilma s perlatora pokazali su visoki stupanj rezistencije prema karbapenemima, cefalosporinima i fluorokinolonima, što je uobičajena karakteristika bolničkih bakterija.
Bakterijski biofilm na perlatorima značajan je rezervoar oportunističkih bakterija te izvor gena rezistencije koji se iz okoliša mogu prenijeti na druge bolničke bakterije. Redoviti mikrobiološki pregled vode/biofilma na perlatorima na odjelima visokog rizika bitan je dio sprječavanja širenja infekcija sa zdravstvenom skrbi. Zbog prisustva oportunističkih bakterija te njihove sposobnosti stvaranja biofilma, neophodno je redovito održavati perlatore na slavinama ili ih češće mijenjati.
Abstract (english) Water in the hospital plumbing premise can serve as a reservoir of potentially pathogenic microorganisms that can cause health-care associated infections. Therefore, 15 samples of water and tap perlators collected in intensive care unit of Clinical Hospital Centre Rijeka were microbiologically analyzed.
Due to the large total colony count, four water samples (27%) were microbiologically unacceptable. Using membrane filtration method, the presence of S. aureus, B. cepacia, L. pneumophila, Aeromonas spp. was excluded from all water samples. Only two samples contained P. aeruginosa (> 103/100 mL). Tap perlators were processed in an ultrasonic bath to desintegrate the biofilm and increase the sensitivity of microbial analysis. Only two of the 14 analyzed perlators cntained no bacterial biofilm, while from the remaining 12 samples, 19 different bacterial strains were detected. Thirteen bacterial isolates belonged to different strains of Pseudomonas aeruginosa, three to Stenotrophomonas maltophilia, and one isolate to Sphingomonas paucimobilis, Alcaligenes faecalis and Enterobacter cloacae. The strains showed a high degree of resistance to carbapenems, cephalosporins and fluoroquinolones, which is a common feature of hospital bacteria.
Bacterial biofilm on perlators represents a significant reservoir of opportunistic bacteria and a source of resistance genes that can be transferred from the environment to other hospital bacteria. Regular microbiological screening of water / biofilms on perlators in high risk units is recommanded. It is imperative to maintain the tap perlators clean or to change them frequently.
Keywords
voda
perlatori
biofilm
bakterije
rezistencija
Keywords (english)
water
tap perlators
biofilm
bacteria
resistance
Language croatian
URN:NBN urn:nbn:hr:184:311241
Study programme Title: Professional study of Medical and Laboratory Diagnostics (Biomedicine and Healthcare; clinical medical sciences) Study programme type: professional Study level: undergraduate Academic / professional title: stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike (stručni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinsko laboratorijske dijagnostike)
Type of resource Text
File origin Born digital
Access conditions Access restricted to students and staff of home institution
Terms of use
Created on 2018-11-20 14:14:37