Knowing and applying assertive communication in everyday life and work would greatly help nurses/technicians (hereinafter referred to as MS/MT) in the satisfaction of the health team's communication and, accordingly, in the quality of work. The aim of this thesis was to examine the level of assertive communication among healthcare workers - nurses/technicians in the Republic of Croatia. 352 respondents of both genders took part in the online survey. The survey lasted two weeks and consisted of socio-demographic questions, a short questionnaire on job satisfaction, stress, and satisfaction with communication among colleagues, and the standardized Rathus Assertiveness Questionnaire, a total of 40 questions. The average level of assertiveness in the overall sample is 4,4±26,102. Men and women did not differ in the average assertiveness score but there is a difference related to educational level. Employees with a higher professional education have a higher level of assertiveness compared to colleagues with a secondary/higher professional education. The following results show that age affects the level of assertiveness, younger MS/MT have a higher level of assertiveness compared to older colleagues. The level of assertiveness of MS/MT has no significance on perceived workplace conflicts, but employees in departments with special working conditions have a higher level of assertiveness than those who work in departments without special working conditions, despite the results showing no statistical significance. It is interesting that MS/MTs with a higher level of assertiveness do not have a higher perceived satisfaction with the conditions and organization of work, nor with communication. The results of this study suggest there is significant room for improvement of communication skills in MS/MT. The implementation of vocational training and education from assertive communication into education and work organizations would contribute to professional personal development as well as better healthcare.