Aim: The main objective of this research is to investigate the peculiarities of physical activity and the connection with mental health among students of health studies in Rijeka. Methods: The research was conducted on a random sample of students attending the Faculty of Health Studies at the University of Rijeka. The questionnaire consists of 4 parts: the 1st part examines general demographic data, the 2nd part consists of a questionnaire for measuring anxiety, depression and stress (DASS-21), the 3rd part includes a scale, i.e. a life satisfaction questionnaire, and the last part 4 consists of a questionnaire on physical activity over the last 7 days (The International Physical Activity Questionnaires, IPAQ). Results: 170 (39.2%) respondents participated in the research, of which the female population predominates, 138 (81.2%), while the average age of the respondents was 28 years. The highest response was recorded by students of graduate university studies in Physiotherapy (N=46, 27.1%) and the most represented place of work was hospitals (N=108, 63.5%). Also, 87 of them (51.8%) stated that they are engaged in some kind of sports activity, and they mostly practice the activities 2-3 times a week (N=51, 58.6%). However, the research found no significant association of physical activity with DASS-21, anxiety, depression and stress, nor with life satisfaction, where the correlation coefficient is weak (rho = -0.135) and not statistically significant (Spearman's correlation test, P = 0.08) . Conclusion: The research did not confirm the fact that physical activity has an impact on mental health, and further research is needed to better examine the relationship between these two variables.