Introduction: Vaccination is one of the most effective and economically most affordable way of protecting the individual and the entire population from certain infectious diseases. The primary goal of vaccination against some disease is its reduction and ultimately its disappearance. In developed countries where there is organized vaccination, certain infectious diseases have been eradicated, while others have significantly reduced their occurrence. The decision on whether a person should vaccinate himself or his/her child is one of the most important and most difficult decisions. There are more and more unproven information available and various conspiracy theories, everyone has the right to freedom of speech, and thus disinformations are easily spreading. Aim: The aim of the final thesis is to collect the knowledge and attitudes of the students of the Faculty of Health Studies, undergraduate study of Nursing on vaccination. Subjects and Methods: The study was conducted on 81 undergraduate Nursing students by an anonymous online survey consisting of 35 questions. The research was approved by the Ethics Committee of the Faculty of Health Studies of the University of Rijeka. Results and Discussion: The research has shown that students of all three years of undergraduate study of Nursing have a positive attitude about vaccination and equal knowledge of the importance of the vaccine coverage of the entire population. First year students have fewer knowledge of the mandatory calendar of vaccination than the third year, and the question on the malaria vaccine best suits the students of the second year, while the third year students gave the worst response. Most of the students have learned about the benefits and the risks of vaccination at the faculty and therefore have access to information by experts and professional literature, and because of the availability of information, most did not independently investigate vaccination, while those who did the research used the Internet and professional literature. Students believe that professional literature is the most accurate source of information. Conclusion: Vaccination is the most important form of protection, and its acceptance and creation of a positive attitude to vaccination is of great importance and largely depends on the education and availability of verified, clear and accurate information to the general population.