Ischemic heart disease is nowadays an unavoidable socio-medical problem, whereas in developed countries it is the leading and the most frequent cause of death among adult population. High sensitivity troponin is the most sensitive marker of myocardial necrosis which is today used in urgent diagnostics. The aim of the thesis is to compare the high sensitivity and the standard method of determining troponins, conduct an analytic verification of high sensitivity method and to demonstrate several examples of measuring troponins resulting in illness. The research included a sample of 40 patients of both sexes within the period of 1st January to 31st December 2015 who were admitted to urgent internal infirmary of Varaždin General Hospital. The research was conducted at the Medical-biochemical laboratory using the analyser of the brand Abbott on Architect ci 8200 platform, which is based on the principle of chemiluminescent biochemical determining using magnetic micro particles. When comparing the high sensitivity method with Passing-Bablok regression analysis on a sample of 38 patients: Y=0,00120000 + 0,950000x (95% CI; A -0,01706 to 1,0729, B 0, 8958 to 1,0729), P=0,26 methods are comparable and Bland-Altman Plot brings the same conclusion. On a sample of 26 patients to 1000 ng/L, Y=0,00293152 + 0,915435 x (95% CI A -0,01000 do 0,003231, B 0,8708 do 0,9650), P=0,77 high sensitivity troponin shows lower results than the standard. The same result is given by the Bland-Altman analysis. Both methods of determining troponins are comparable, although the high sensitivity method is more sensitive, as expected. Through a conducted analytic verification of highly sensitive troponin I we have proven that the laboratory gives reliable results. The change of value of the high sensitivity troponin is important for excluding or confirming the myocardial necrosis. High sensitivity method of determining troponins can also be a very important diagnostic tool in anticipating future risks among healthy patients.