The seismic performance assessment of heritage architecture presents many challenges due to the restrictions set forth by the conservation principles to protect the associated social and cultural values. These buildings are typically characterized by unreinforced masonry walls connected to-gether by tie-rods, vaults, and wooden floors. The era of construction dates to the time when seismic design regulations were largely unknown, making the heritage structures potentially vul-nerable to earthquake damage. This study presents the seismic performance assessment of the Jes-uit College located in the southern part of the Old City of Dubrovnik. A series of field surveys were conducted to qualitatively examine the material composition and obtain geometrical details in part of the Croatian Science Foundation research project IP-2020-02- 3531 entitled “Seismic Risk Assessment of Cultural Heritage in Croatia - SeisRICHerCRO”. The structural response is thor-oughly investigated by means of a complex finite element model calibrated using the frequencies determined from ambient vibration measurements, and material characteristics obtained from the literature review of representative cultural heritage buildings. The seismic performance is evalu-ated using linear static and response spectrum analysis in accordance with Eurocode 8 guidelines for the demand seismic action level. The numerical analysis indicates several structural compo-nents in the building exhibiting high shear stress concentration and exceeding the elastic tensile limit under the demand ground acceleration level. The assessment further reveals substantial out-of- plane bending of vulnerable wall components (identified by local mode shapes) at low peak ground acceleration levels. The stress concentration in numerous structural components leads to the identification of vulnerable zones where retrofitting measures are essentially required.