The subject of master thesis are issues and challenges of macrophotography, which are occurred accompanied by category of digital photography shooting. For understanding the subject it is necessary to know basic terms of photography. Physical shooting phenomena and limitations that determinate shooting are explained in detail. In this labor separately will be presented equipment that is required for macrophotography and equipment like lens and tripod that is frequently used in this type of photography. Different types of settings are examined, such as focusing, depth of field and natural or artificial light. There are also examined typical digital processing of photography and its effect in the final quality of the photography, for example computer correction and sharpness editing. Targeted macro-photos are shooted based on knowledge of theoretic analyze in digital photographic system and then analyzed and digital formatted. There are also elaborated techniques, of digital photographic system and possibility of its use in field of macrophotography, from technical and creative aspect. This thesis attempts to explore the area of macro photography with the practical side and less with detailed clarifying technology of mentioned field.