Modern systems of visual communications, on which reproduction processes of graphic technology are also based on, are permanently improved with new findings in conjuction with the research of certain visual effects. As a part of this thesis, the influence of various parameters within the target process of graphic reproduction on the intensity and appearance of the neon colour spreading effect was investigated. The influence of different media (two printing substrates, and a computer screen) in the “cross-media” reproduction system of the graphic products production was examened, as well as the role of the inserted segment color choice and background of the primary stimulus (different combinations of primary colors additive and subtractive synthesis) as an element of design solutions. These parameters were evaluated in a variety of ambient conditions and under the observation of three CIE standard light sources and illuminants. Neon colour spreading effect manifests itself in the shift of colour appearance by creating apparent colour spreading of the primary stimulus in the background of graphic reproductions, in the lattice as large as the primary stimulus, but also in the process of pattern shaping while providing design solutions. The study of effect was conducted on the test samples, based on Ehrenstein figure, i. e. specific geometric structure consisting of sets of orthogonal lines formed by combining various color combinations of the primary stimuli. The intensity of the effect is calculated using the color difference variable E94 , which represents the deviation in manifestation of neon colour spreading effect in relation to the color of the reproduction media. The shift in color appearance of the neon colour spreading effect is described through the calculation of changes in perceptual attributes of color, i. e. the lightness difference L* , the chroma difference * ab C and the hue difference * ab H . Statistical analysis of results was conducted by using appropriate nonparametric tests. Based on the obtained results, the guidelines for the future work are given, mainly about how to apply examined influential parameters in processes of designing and printing graphic products, having in mind potential manifestation of the neon colour spreading effect.