Morningness-Eveningness preference or circadian typology (morning type, neutral type, evening type) is individual difference in circadian expression of biological and bihevoral functions which significantly influences individual everyday functioning and quality of life. The aim of this study way to examine relationship between Morningness-Eveniningess and personality traits in broad and narrow sense across three generations. There were total of 199 participants in this research, of which were 133 students (89,5% female), 36 parents (50% female) and 30 grandparents (50% female). The participants filled questionnare which contained demographic characteristics and two starndardized instruments – International Personality Item Pool 50 (IPIP 50) and Composite scale of Morningness (CSM). Statistically significant difference in Morningness-Eveningness was discovered across three generations. Generation of students contained more evening types than generations of parents and grandparents, where there were no evening types in generation of grandparents. There were no gender differences in Morningness-Eveningness. There was statistically significant positive correlation between morningness and Conscientiousness, negative correlation between morningess and Extraversion and negative correlation between morningness and Intelect when whole sample was included. Statistically significant positive correlation between morningness and Conscientiousness was found in student sample. In generation of parents statistically significant negative correlation between morningness and Emotional Stability was discovered, whereas correlation between morningness and Emotional Stability proved to be statistically positive in grandparents generation. Statistically significant positive correlation between morningness and Conscientiousness was found in generation of grandparents. Obtained results give us an insight into relationship between Morningness-Eveningness and personality traits in our country which was missing and bring us new result about relationship between morningess and Intelect, which needs further investigation.