Workplace stress is the primary problem of the European Agency for Safety and Health at work. Healthcare system is labour intense and economically one of the largest sectors of the European Union, and healthcare professionals have been recognized as a profession that is highly stressful. This research aims to identify and assess the intensity of stress factors at workplace with nurses and technicians employed in intensive care units, surgery, emergency medicine and oncology department to enable steps toward prevention. In assessing the experience of stress Questionnaire about stressors in the workplace for hospital healthcare workers was used. The study was conducted on a sample of 153 participants using online survey. Overall experience of stress is borderline high. The experience of stress is high on the subscales "Organisation and Finance" and "Public criticism and lawsuits." Participants feel quite physical fatigue and tension and distinct mental fatigue even after normal working day. Nursing staff sometimes thinks of leaving job, but they are quite satisfied with their work and believe that they would be able to do the same job in two years. We have detected differences in the overall experience of stress considering the area of work. Statistically significant differences in the experience of stress considering the area of work were found on subscales "Organisation and Finance", "Public criticism and litigation", "Shift Work" and "Professional and intellectual requirements." There was no correlation between the length of the work in this workplace and overall experience of stress. There was a low negative correlation between overall stress experience and overall job satisfaction and overall stress experience and predictions of participants about the possibility of doing the same job for two years. In the end, some shortcomings, but also some benefits of this research, are given, and suggestions for future research and measures of prevention and intervention based on the implications of results are presented.