The subject of employee motivation and satisfaction becomes one of the main questions in recent time due to the lack of quantity and qualified employees in tourism. The objective of this paper is to find what is job satisfaction level of cruise ships employees’ and significance of chosen satisfaction factors in order to provide inputs that could help the companies in motivation efforts to achieve higher level of job satisfaction. The quantitative research was conducted by the questionary on 269 cruise ship employees from 19 different countries in 2022. The analysis was done for all participants in general and additionally to gender and dividing participants to service staff and managerial staff. Two main tests were used: Student’s two sample t-test of independent samples and Two sample z-test of proportions. It was found, in general, that cruise ships employees show the highest degree of satisfaction with factors such are paid expenses, destination, safety of job, independence in working, and flexibility and creativity. The lowest degree of satisfaction was showed by working hours, variable salary such are bonuses, commissions and incentives, and possibility of getting good tips. The managers have shown the highest level of satisfaction degree with paid expenses, destination, basic salary, safety of job and independence in work. The service staff have shown the similar satisfaction. The satisfaction with tips was the only factor for which was found statistically significant difference between managerial and service employees. There is statistically significant difference in non-satisfaction of male and female employees, for example, in safety of job, receiving feedback on work performance, ability of supervisor to recognize good job and appreciation of their opinions and suggestions for business improvement. The female employees have shown the higher level of dissatisfaction with those factors. Looking at high levels of satisfaction there was no statistically significant difference between male and female employees, except for appreciation of their opinions and suggestions for business improvement by supervisor. The male employees were more satisfied with this factor. The results of this paper are significant to the theory and practice, as there are a very few researches on cruise employees’, even in general.