Dataset: shaping_the_gut_microbiota.zip, 15.02 MB Access Condition: Open access Description: In vitro experiments: Bifidogenic and lactogenic effect. In vivo experiments: metagenomic analyses, microbiological analyses (English)
Jerić, I. (2024). Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP) [Data set]. https://urn.nsk.hr/urn:nbn:hr:241:813036.
Jerić, Ivanka. Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP). Institut Ruđer Bošković, 2024. 12 Sep 2024. https://urn.nsk.hr/urn:nbn:hr:241:813036.
Jerić, Ivanka. 2024. Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP). Institut Ruđer Bošković. https://urn.nsk.hr/urn:nbn:hr:241:813036.
Jerić, I. 2024. Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP). Institut Ruđer Bošković. [Online]. [Accessed 12 September 2024]. Available from: https://urn.nsk.hr/urn:nbn:hr:241:813036.
Jerić I. Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP). [Internet]. Institut Ruđer Bošković: Zagreb, HR; 2024, [cited 2024 September 12] Available from: https://urn.nsk.hr/urn:nbn:hr:241:813036.
I. Jerić, Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP), Institut Ruđer Bošković, 2024. Accessed on: Sep 12, 2024. Available: https://urn.nsk.hr/urn:nbn:hr:241:813036.
Title (english)
Dataset for metagenomic and microbiological analysis of Food for Special Medical Purposes (FSMP)
Food for Special Medical Purposes (FSMP) is a class of therapeutic products specifically formulated for the dietary management of diseases, disorders or conditions in patients. The consumption of dietary fibres that can be used as components of FSMP may have a strong influence on the composition of the gut microbiota. Therefore, the aim of this paper was to examine innovative mixture of dietary fibres in FSMP in order to modulate the imbalanced gut microbiome of the tumour-induced melanoma mouse model. Five different dietary fibres, fructooligosaccharides, long-chain inulin, corn dextrin, gum arabic and citrus fibre, were selected as an innovative dietary fibre mixture designed for oncological medical purpose based on their lactogenic and bifidogenic effects obtained by in vitro experiments. Based on metagenomic analyses of the gut oncomicrobiome of experimental oncological mice, a prebiotic effect was displayed in vivo after administration of the formulated dietary fibre mixture, particularly in terms of an enrichment of Bifidobacterium pseudolongum, the strongest predictor genus for the prebiotic effect in the post-fibre gut oncomicrobiomes. An enrichment of favourable gut microbiota species with health-promoting benefits and a depletion of unfavourable gut bacteria were also observed during the application of chemotherapy in tumour-induced mice, with an additional enrichment of favourable gut microbiota species. This suggests that the selected dietary fibres can be used to improve the overall balance of the gut microbiome as an additional intervention in cancer therapy.
Methods (english)
The lactogenic and bifidogenic effects of dietary fibres were determined from faecal samples collected from male and female healthy mice.
In vivo experiments were performed on 3-month-old experimental mice of the black strain C57 weighing 20 to 25 g.
The MRS agar medium was used to determine the number of bacterial colonies of the Lactobacillus species, i.e. the lactogenic effect. The TOS propionate agar medium was used to determine the number of bacteria of the genus Bifidobacterium, i.e. the bifidogenic effect.