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Scientific paper - Original scientific paper
Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?
Brain Injury, 30 (2016), 11; 1390-6. https://doi.org/10.1080/02699052.2016.1195518
Mišir, Mihael; Renić, Marija; Mihalj, Martina; Novak, Sanja; Drenjančević, Ines

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Mišir, M., Renić, M., Mihalj, M., Novak, S. & Drenjančević, I. (2016). Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?. Brain Injury, 30. (11), 1390-6. doi: 10.1080/02699052.2016.1195518

Mišir, Mihael, et al. "Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?." Brain Injury, vol. 30, no. 11, 2016, pp. 1390-6. https://doi.org/10.1080/02699052.2016.1195518

Mišir, Mihael, Marija Renić, Martina Mihalj, Sanja Novak and Ines Drenjančević. "Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?." Brain Injury 30, no. 11 (2016): 1390-6. https://doi.org/10.1080/02699052.2016.1195518

Mišir, M., et al. (2016) 'Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?', Brain Injury, 30(11), pp. 1390-6. doi: 10.1080/02699052.2016.1195518

Mišir M, Renić M, Mihalj M, Novak S, Drenjančević I. Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?. Brain Injury [Internet]. 2016 [cited 2024 November 26];30(11):1390-6. doi: 10.1080/02699052.2016.1195518

M. Mišir, M. Renić, M. Mihalj, S. Novak and I. Drenjančević, "Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?", Brain Injury, vol. 30, no. 11, pp. 1390-6, 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:239:717761. [Accessed: 26 November 2024]

Metadata
Title (english)Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?
AuthorMihael Mišir
MBZ: 357666
AuthorMarija Renić
MBZ: 194961
AuthorMartina Mihalj
MBZ: 322792
AuthorSanja Novak
MBZ: 326355
AuthorInes Drenjančević
MBZ: 209603
Author's institution(Clinical hospital center Osijek)
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract (english)
AIM: To determine optimal duration of transient middle cerebral artery occlusion (t-MCAO) for a stroke model in female diabetic Sprague-Dawley (SD) rats. METHODS: Streptozotocin-induced type-1 diabetic SD female rats (n = 25, 12 weeks old, five groups; n = 5 per group) were subjected to different duration of t-MCAO (20, 30, 45, 60 and 90 minutes) followed by reperfusion. A control group of rats without diabetes (n = 5) was subjected to 30 minutes of t-MCAO followed by reperfusion. Twenty-four hours after reperfusion, infarct volumes were evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining. RESULTS: Intra-ischaemic reductions of regional cerebral blood flow (rCBF) were similar in all groups (68-75% of baseline values). Reperfusion was significantly impaired in the 90-minute ischaemia group (56-62% vs 80-125% in other groups). Twenty minutes of t-MCAO induced a small infarct (3 ± 5% of ischaemic hemisphere). Thirty minutes of ischaemia produced a significantly larger infarct (46 ± 6%). In the 45 and 60 minute groups, ischaemia infarct was 52 ± 5% and 59 ± 3% of the ischaemic hemisphere, respectively. Ischaemia of 90' led to a massive stroke (89 ± 6% of ischaemic hemisphere encompassing the whole striatum (22 ± 3%) and almost the whole MCA irrigated cortex area (67 ± 6%)). Thirty minutes of t-MCAO did not produce stroke in the control group. CONCLUSION: The diabetic rat stroke model should be different from the non-diabetic, because female type-1 diabetic SD rats are highly sensitive to brain ischaemia and it is necessary to significantly shorten the duration of t-MCAO, optimally to 30 minutes.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBrain Injury
Numberingvol. 30, no. 11, pp. 1390-6
p-ISSN0269-9052
e-ISSN1362-301X
DOIhttps://doi.org/10.1080/02699052.2016.1195518
URN:NBNurn:nbn:hr:239:717761
Publication2016
Document URLhttps://www.tandfonline.com/doi/full/10.1080/02699052.2016.1195518
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-01-20 12:53:44