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undergraduate thesis
Nove metode sekvenciranja DNA
Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2018. urn:nbn:hr:182:829180
Trupković, Rene
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

Institutional repository: Repository of the Department of Chemistry, Osijek

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Trupković, R. (2018). Nove metode sekvenciranja DNA (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:829180

Trupković, Rene. "Nove metode sekvenciranja DNA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:182:829180

Trupković, Rene. "Nove metode sekvenciranja DNA." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:182:829180

Trupković, R. (2018). 'Nove metode sekvenciranja DNA', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:182:829180

Trupković R. Nove metode sekvenciranja DNA [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2018 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:182:829180

R. Trupković, "Nove metode sekvenciranja DNA", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:182:829180

Metadata
TitleNove metode sekvenciranja DNA
Title (english)New DNA sequencing methods
AuthorRene Trupković
MentorMartina Šrajer Gajdošik (mentor)
MentorAna Amić (pomoć pri izradi, neposredni voditelj)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2018-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Sekvenciranje DNA je metoda koja omogućava određivanje redoslijeda nukleotida u DNA molekuli. Metode se dijele na tri generacije, koje se razlikuju po brzini, preciznosti, propusnosti, veličini fragmenata, te načinu njihove detekcije i analize. 1977. godine razvijena je Sangerova metoda, temeljena na sekvenciranju DNA fragmenata pomoću prethodno obilježenih dideoksinukleotid trifosfata koji sprječavaju povezivanje daljnjih nukleotida i time prekidaju sintezu lanca DNA. Paralelno sa Sangerovim otkrićem, 1977. godine Maxam i Gilbert razvijaju metodu temeljenu na nasumičnom cijepanju fragmenata DNA na različitim bazama s obzirom na korišteni agens (hidrazin, dimetil sulfid i dr.). Razvojem tehnologije dolazi do pojave metoda druge generacije kao što je pirosekvenciranje u kojem prilikom polimerizacije DNA dolazi do otpuštanja pirofosfata i emitiranja fotona koji služe za detekciju fragmenata. Pojavom detekcije u stvarnom vremenu otvorene su nove mogućnosti sekvenciranja DNA, poput monomolekulskog sekvenciranja (SMRT), te sekvenciranje pomoću nanopora. Sukladno razvoju novih metoda, otkrivene su nove tehnike sekvenciranja poput 454 pirosekvenciranja, Illumina, SOLiD, te HeliScopea. Korištenje metoda sekvenciranja DNA omogućava napredak brojnih prirodnih znanosti, poput genetike, biomedicine i biokemije, ali i napredak u proučavanju raznih bolesti, kao i u razvoju novih lijekova.
Abstract (english)
DNA sequencing is a method that allows determination of nucleotide order in DNA molecule. Methods are divided into three generations, which differ in speed, precision, throughput, fragment size, and detection and analysis. In 1977, Sanger et al. developed a method based on DNA sequencing by pre-labelled dideoxynucleotide triphosphates, which prevent incorporation of further nucleotides and interrupt synthesis of DNA chain. In 1977, Maxam and Gilbert developed another method based on random cleavage of fragments depending on the used agents (hydrazine, dimethyl sulfide, etc.). Advancement of technology led to the development of the second generation methods, such as pyrosequencing in which DNA polymerization results in the release of pyrophosphates and emission of photons, used for the detection of fragments. Real-time detection opens new possibilities for DNA sequencing, such as single molecule real time sequencing (SMRT) and nanopore sequencing. In accordance with the development of new methods, new sequencing techniques, such as 454 pyrosequencing, Illumina, SOLiD and HeliScope, are discovered. The use of DNA sequencing allows advancement of numerous natural sciences, such as genetics, biomedicine and biochemistry, as well as a progress in studying of various diseases and development of novel drugs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:829180
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-12-13 11:29:25