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master's thesis
Utjecaj stresa na ekspresiju fotosintetskih proteina
Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. urn:nbn:hr:182:694477
Musa, Matea
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

Institutional repository: Repository of the Department of Chemistry, Osijek

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Musa, M. (2019). Utjecaj stresa na ekspresiju fotosintetskih proteina (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:694477

Musa, Matea. "Utjecaj stresa na ekspresiju fotosintetskih proteina." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:694477

Musa, Matea. "Utjecaj stresa na ekspresiju fotosintetskih proteina." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:694477

Musa, M. (2019). 'Utjecaj stresa na ekspresiju fotosintetskih proteina', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:182:694477

Musa M. Utjecaj stresa na ekspresiju fotosintetskih proteina [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2019 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:182:694477

M. Musa, "Utjecaj stresa na ekspresiju fotosintetskih proteina", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:182:694477

Metadata
TitleUtjecaj stresa na ekspresiju fotosintetskih proteina
Title (english)Effect of stress on the expression of photosynthetic proteins
AuthorMatea Musa
MentorMartina Šrajer Gajdošik (mentor)
MentorSelma Mlinarić (komentor)
Committee memberAna Amić (predsjednik povjerenstva)
Committee memberMartina Šrajer Gajdošik (član povjerenstva)
Committee memberSelma Mlinarić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2019-10-04, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom radu je istraživan utjecaj stresa sušom i visokim intenzitetom svjetlosti na ekspresiju D1 podjedinice fotosustava II (PSII), PsbO podjedinice kisik razvijajućeg kompleksa (OEC) i velike podjedinice (LSU) enzima Rubisco u klijancima ječma (Hordeum vulgare L.) kultivara Panonac. Klijanci ječma su izloženi stresu izazvanom sušom u trajanju od 14 dana te visokom intenzitetu svjetlosti u trajanju od 4, odnosno 11 sati u klima komori. Kako bi se odredila količina ispitivanih fotosintetskih proteina, proteini su razdvojeni denaturirajućom elektroforezom u poliakrilamidnom gelu (SDS-PAGE) nakon čega su prenešeni na nitroceluloznu membranu (Western blot) te detektirani specifičnim protutijelima. Izlaganje stresu izazvanom sušom nije doveo do snažnije degradacije sva tri ispitivana fotosintetska proteina. Izlaganje stresu uzrokovanom visokim intenzitetom svjetlosti pokazao je snažniju degradaciju u odnosu na izlaganje suši za sva tri ispitivana proteina. Kraće izlaganje visokom intenzitetu svjetlosti (4h) izazvalo je jaču degradaciju D1 i LSU podjedinice u odnosu na dugotrajnije izlaganje (11h) ovom tipu stresa. Dobiveni rezultati upućuju na to da je izabrana sorta ječma pokazala manju osjetljivost na sušu u odnosu na visoki intenzitet svjetlosti.
Abstract (english)
In this thesis, the effect of stress, trough drought and high light intensity, on the expression of D1 subunit of photosystem II, the PsbO subunit of the oxygen-evolving complex (OEC), and the large subunit (LSU) of the Rubisco enzyme in barley (Hordeum vulgare L.) cultivar Panonac was investigated. e. Barley seedlings were exposed to 14 days of drought stress and to high light intensity stress in the climate chamber in duration of 4 and 11 hours. To determine the amount of examined photosynthetic proteins, denaturing polyacrylamide gel electrophoresis (SDS-PAGE) was used for their separation followed by the transfer to a nitrocellulose membrane (Western blot) and detection with specific antibodies. Exposure to drought induced stress did not result in significant degradation of any of three examined photosynthetic proteins. Exposure to stress caused by high light intensity showed stronger degradation compared to drought exposure in all three proteins. Shorter exposure to high light intensity (4h) caused more degradation of the D1 and LSU subunits when compared to a longer exposure (11h) to this type of stress. The results obtained indicate that the selected cultivar of barley showed a lower sensitivity to drought compared to the high light intensity stress.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:694477
Study programmeTitle: Graduate University Study of Chemistry; research direction
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-15 10:00:08