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master's thesis
Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu
Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. urn:nbn:hr:182:380089
Andabak, Marija
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

Institutional repository: Repository of the Department of Chemistry, Osijek

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Andabak, M. (2019). Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:380089

Andabak, Marija. "Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:380089

Andabak, Marija. "Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:380089

Andabak, M. (2019). 'Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:182:380089

Andabak M. Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2019 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:182:380089

M. Andabak, "Određivanje metalotioneina elektroforezom u poliakrilamidnom gelu", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:182:380089

Metadata
TitleOdređivanje metalotioneina elektroforezom u poliakrilamidnom gelu
Title (english)Metallothionein detection in polyacrylamide gel by electrophoresis
AuthorMarija Andabak
MentorMartina Šrajer Gajdošik (mentor)
Committee memberAna Amić (predsjednik povjerenstva)
Committee memberMartina Šrajer Gajdošik (član povjerenstva)
Committee memberOlivera Galović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2019-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Metalotioneini (MT) su mali peptidi prisutni u stanicama mnogih organizama. Zbog male molekulske mase i specifične strukture, koju čini jedan polipeptidni lanac bogat cisteinom i metalni ioni vezani u njegovom središtu, njihova detekcija i kvantifikacija predstavljaju izazov. Elektroforeza u poliakrilamidnom gelu je jednostavna i dostupna metoda za određivanje proteina kompleksnih bioloških uzoraka. Pruža mogućnost optimizacije uvjeta u kojima se izvodi, čime se omogućuje razdvajanje i vrlo malih proteina. Cilj ovog rada je pronaći najbolje uvjete za određivanje metalotioneina u uzorcima tkiva gujavica (Lumbricus terrestris) elektroforezom u poliakrilamidnom gelu. Eksperimentalno je provjeren utjecaj različitih čimbenika na uspješnost elektroforetskog razdvajanja metalotioneina, kao što su: različita priprema uzoraka, koncentracija poliakrilamidnog gela, derivatizacija uzoraka monobromobimanom (mBBr) te puferski sustavi u kojima se elektroforeza izvodi. Iako je elektroforezom u poliakrilamidnom gelu moguće detektirati MT, prethodno pročišćavanje i frakcioniranje uzoraka nužno je za uspješnu analizu.
Abstract (english)
Metallothioneins (MTs) are small peptides found in cells of wide range of living organisms. Their detection and quantification present challenge owing to their small molecular mass and specific structure. MTs structure is simple, consisted of one polypeptide chain rich in cysteine, and metal ions bound in the center of that chain. Electrophoresis in polyacrylamide gel is simple and available method for protein detection in complex biological samples. Since its conditions can be altered and optimized, even very small proteins can be detected by this method. The aim of this paper is to find best conditions for MTs detection in earthworm (Lumbricus terrestris) tissue by electrophoresis in polyacrylamide gel. Many different factors that can affect efficacy of electrophoresis were experimentally verified: different sample preparation, concentration of polyacrylamide gel, samples derivatization with monobromobimane (mBBr) and buffers in electrophoretic system. Although MTs can be detected by electrophoresis in polyacrylamide gel, purification and fractionating of samples are necessary for successful analysis and better results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:380089
Study programmeTitle: Graduate University Study of Chemistry; research direction
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-20 09:38:47