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master's thesis
Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima
Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2024. urn:nbn:hr:182:861136
Šimić, Marko
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

Institutional repository: Repository of the Department of Chemistry, Osijek

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Šimić, M. (2024). Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:861136

Šimić, Marko. "Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:182:861136

Šimić, Marko. "Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2024. https://urn.nsk.hr/urn:nbn:hr:182:861136

Šimić, M. (2024). 'Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:182:861136

Šimić M. Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2024 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:182:861136

M. Šimić, "Promjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:182:861136

Metadata
TitlePromjene u proteomu Yersinie enterocolitica nakon tretiranja kvaternim amonijevim spojevima
Title (english)Changes in the proteome of Yersinia enterocolitica after treatment with quaternary ammonium compounds
AuthorMarko Šimić
MentorMartina Šrajer Gajdošik (mentor)
Committee memberTomislav Balić (predsjednik povjerenstva)
Committee memberMartina Šrajer Gajdošik (član povjerenstva)
Committee memberOlivera Galović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2024-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Applied Chemistry
Abstract
Bakterija Yersinia enterocolitica, gram-negativni patogen koji se esto prenosi hranom, testirana je s dezinficijensima na bazi kvaternih amonijevih soli. U testiranju su koribtene prethodno sintetizirane kvaterne amonijeve soli s kloridnim anionom kao inhibitori. Promjene u proteomu analizirane su metodama koje koriste gelove i metodama bez gelova. Klju na uloga pripreme uzoraka posebno se isti e u proteoliti koj digestiji. Daljnje analize provedene su teku inskom kromatografijom povezanom sa spektrometrijom masa, a dobiveni podatci odrađeni su putem alata QuickGO i UniProt. Kona na analiza pokazala je razlike u koli inama „up“ i „down“ reguliranih proteina, omogu uju i uvid u promjene proteoma i klju ne proteina za rast, razvoj i pre~ivljavanje bakterije pod stresom.
Abstract (english)
The bacterium Yersinia enterocolitica, a gram-negative pathogen frequently transmitted through food, was tested with disinfectants based on quaternary ammonium compounds. In the testing, previously synthesized quaternary ammonium compounds with chlorides were used as inhibitors. Proteome changes were analyzed using both gel-based and gel-free methods. The crucial role of sample preparation is particularly highlighted in proteolytic digestion. Further analyses were conducted using liquid chromatography coupled with mass spectrometry, and the obtained data were processed using QuickGO and UniProt tools. The final analysis showed differences in the quantities of up- and down-regulated proteins, providing insight into proteome changes and key proteins involved in the growth, development, and survival of the bacterium under stress.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:861136
Study programmeTitle: University Graduate Study of Chemistry; Research Program; specializations in: Research Program
Course: Research Program
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra kemije (sveučilišni/a magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-03 10:12:44