Genome research, a set of data that together with environmental factors determines the abilities and characteristics of an individual, represents the greatest progress and challenge in modern science. In the last thirty years, we have witnessed great progress in genome research at all levels. In the context of sports, the association of more than 200 gene polymorphisms with motor and functional abilities has been confirmed. The purpose of this research was to determine the occurrence of genotypes of the R577X polymorphism in the ACTN3 gene, the Trp64Arg polymorphism in the ADRB3 gene, and the insertion-deletion (Ins-Del) polymorphism of the ACE gene in players of the highest competitive team sports in Croatia. For the aforementioned gene polymorphisms, previous research has indicated a possible connection with success in energy-extreme sports such as power lifting and sprinting and, on the other hand, with marathons and sports with a high dominance of aerobic endurance. Data on the influence of the mentioned genes in team sports in previous research were inconsistent. The sample consisted of 56 soccer players, 40 basketball players and 31 volleyball players. The presumed superior RR genotype for explosiveness and strength of the R577X ACTN3 gene polymorphism was present in 37% of individuals in a selected sample of elite athletes. The research showed that there are statistically significant differences in the distribution of the RR genotype of the ACTN3 gene polymorphism between top athletes of all three sports that we analyzed compared to the unselected general population from the work of Massad et al., 2015, which was originally taken as a reference of the control unselected general population. On the other hand, the difference was not significant between the selected population of top athletes in this paper and the general population from a recent study, which geographically ideally corresponded to the population of top athletes in the Republic of Croatia from this paper (Konakli et al., 2017). An increased frequency of the Ins /Ins ACE gene genotype or the Trp/Trp and Trp/Arg ADRB3 gene genotypes was not observed in the elite athletes of our study, which were assumed to be more frequent in sports with an emphasized aerobic component. The results indicated statistically significant differences in the 'genetic sports index' between football players in midfield and attacking positions. Based on the obtained data, it was concluded that there is no statistically significant difference in the incidence of studied polymorphisms between team sports athletes and the general population of this region. This research raises the question of the ability to asses the coefficient of influence of gene polymorphisms and their interactions on sport abilities and skills that determine the sports success.