Handball is a global sport that belongs to the group of aerobic-anaerobic sports. Often referred to as a transition game because of the frequent changes in defense and attack, this game is characterized by frequent high-intensity contact with intermittent runs and sprints. Endurance is one of the dominant motor abilities in the hierarchical structure of success in handball. Therefore, research into the status of handball players in that area is of great interest to handball experts. 51 female handball players from the same handball club (age 17.39 ± 4.04 years, height 172.73 ± 7.93 cm and body weight 64.57 ± 10.18 kg) were divided into 3 age categories: seniors, cadets and junior cadets. 17 senior women (age 22.35 ± 2.78 years, body height 175.88 ± 6.55 and body mass 71.65 ± 10.80), 14 female cadets (height 171.46 ± 9.26 cm and body mass 64.64 ± 6.63 kg) and 20 younger female cadets (height 170.93 ± 7.57 cm and body weight 58.52 ± 7.82 kg) participated in the 30-15 Intermittent Fitness Test. The last running speed on the 30-15 Intermittent Fitness Test was recorded and, along with other anthropometric measurements, entered into the algorithm for the assessment of maximum oxygen intake. The obtained data were analyzed with descriptive statistics (arithmetic mean, standard deviation, minimum and maximum score), then the normality of the distribution of variables was analyzed (Kolmogorov-Smirnov test). In accordance with the determined distribution, adequate parametric methods (ANOVA) and non-parametric methods (Kruskal-Wallis test) of data processing were applied to determine the differences between the 3 groups of handball players. A statistically significant difference between the groups of handball players was found in the morphological variable of body mass and the estimated maximum oxygen intake (VO2max), while there were no statistically significant differences in the variables of body height and in the variable of the final speed run on the 30-15 Intermittent Fitness Test (vIFT).