Post-Activation Performance Enhancement (PAPE) is a phenomenon which leads to muscle strength and performance increasement following a high-intensity stimulus in the form of a loaded exercise which is biomechanically similar to the movement being potentiated. Its impact on voluntary muscle failure has not been clearly established, especially in the female population. Therefore, the aim of this study is to define the impact of PAPE (90% of 1 maximum repetition, 1RM) on voluntary muscle failure during the leg press exercise (at 70% of 1RM) through the number of repetitions in different sets, and thus on the total training volume among women. The sample included 14 women (age 23.5 ± 1.9 years; body mass 61.4 ± 7.9 kg; body height 165 ± 6.2 cm) without musculoskeletal injuries or other health problems, with an average leg press 1RM of 155 ± 31.9 kg and reported resistance training experience of 5.5 ± 3.3 years. The research protocol consisted of three visits. During the first visit, the 1RM was tested. For the subsequent two visits, participants were randomly divided into two groups based on the alphabetical order of their last names. These visits included a control session (CON) and an experimental session (PAPE), during which participants performed three working sets to failure at 70% of their 1RM after an 8-minute rest. The results did not show a statistically significant difference in the number of repetitions in the first and second sets and the total volume, while the difference in the third working set was statistically significant (p = 0.037). However, it is questionable whether this result can be attributed to PAPE considering the long rest periods between sets and the potentiation duration of PAPE. The heterogeneity of the participants' training experience is a potential reason for the dispersion and thus the inconsistency of the results compared to previous studies, and it is advised to individualize rest periods in future studies. Although gender should not play a significant role in the outcomes, women may experience fewer benefits from PAPE regarding voluntary muscle failure.