In today's modern world, awarness of the importance and benefits of physical activity is still not at a satisfactory level, while on the other hand, we have increasing shares of sedentary behavior, especially among children and young people, mainly due to the modernization and technological progress of society. The benefits of physical activity are manifested in lowering blood pressure, improving insulin sensitivity, better sleep, stress regulation, cognitive functions, etc. The aim of this research is to analyze physical activity and sedentary behavior of female and male students of the Faculty of Kineisology and the Faculty of Agriculture of the University of Zagreb. A total of 250 students, with an average age of 20,7 years, filled out the KINAKT questionnaire in the form of a Google form consisting of 18 questions and a total of 53 items. The Mann-Whitney U-test was used to determine the differences between the students of the two faculties in the level of physical activity and sedentary behavior, as well as differences by gender, at the level of error of statistical inference p˂0,05. A statistically significant difference in physical activity was obtained where students of the Faculty of Kinesiology are more active than students of the Faculty of Agriculture, and male students of both faculties are more active than the female students. In the case of sedentary behavior, the differences between students with regard to faculty and gender are statistically significant in three out of 10 observed variables. Among the faculties, it is about variables that examine how much students on average per day spend studying, walking around shopping centres and in front of the computer for studying. By gender, the differences are statistically significant for the first two aforementioned variables, while the third relates to how much they spend on average per day in front of the computer for entertainment.