The study was conducted to determine the difference between water polo players of different age groups in individual motor and functional abilities and the water polo specific polygon. Four anthropometric measurements, four waterpolo specific test and three complex (polygon) water polo test were used on the 76 water polo players. All tests were conducted three times apart from polygons that were made twice. The results of the study showed statistically significant differences in anthropometric variables between younger juniors and other tested groups. Swing and maximum swim variations showed a significant difference between younger juniors and other groups, while the striking impact variable and maximum dynamometer showed the difference between all the tested groups. Each of the 3 tested polygons had different results. Thus, a striker ending a goal shows a significant difference between all groups, a polygon that ends with a distinct difference between younger juniors and other groups, while the pitch variation showed the difference between seniors and other groups, but no distinction was found between young juniors and juniors. By analyzing the complete results we can conclude that water polo players in the age of younger juniors are still in the phase of accelerated growth and development while the juniors are very similar to the senior in morphological traits. Variables where the most explosive power (shots and maximum dynamometers, polygon shots) are most revealing indicate a significant difference between the seniors and other groups. We hope that water polo trainers will respect the biological development of the individual and that the conclusions of this research will be applied in the planning and programming of the training process, especially in younger ages.