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master's thesis
GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2022. urn:nbn:hr:167:309494
Šunjerga, Antonia
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Institutional repository: Repository of the Faculty of chemistry and technology

Cite this document

Šunjerga, A. (2022). GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:309494

Šunjerga, Antonia. "GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:309494

Šunjerga, Antonia. "GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:309494

Šunjerga, A. (2022). 'GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:309494

Šunjerga A. GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2022 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:167:309494

A. Šunjerga, "GC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:167:309494

Metadata
TitleGC-MS temeljna komparativna metabolomika dviju staničnih linija raka dojke tretiranih nootkatonom : diplomski rad
Title (english)GC-MS based comparative metabolomies of two breast cancer cell lines treated with nootkatone : master thesis
AuthorAntonia Šunjerga
MentorMila Radan (mentor)
Committee memberMladen Miloš (predsjednik povjerenstva)
Committee memberMarina Zekić (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2022-10-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Proliferacija, diferencijacija i preživljenje pojedinačne stanice u višestaničnom je organizmu pažljivo regulirano, dok u stanicama raka te regulacije nema, one nekontrolirano rastu i dijele se, šire po čitavom tijelu i ometaju funkciju normalnih tkiva i organa. Rak je vodeći uzrok smrti u svijetu. Najvažnije u patologiji raka jest razlikovati benigne (dobroćudne) od malignih (zloćudnih) tumora. Drugi način klasifikacije je prema tipu stanica od kojih je rak sastavljen, prema čemu su glavne podvrste: karcinomi, sarkomi, limfomi, leukemije i mijelomi. Rak dojke je vodeći uzrok smrti povezanih s rakom među ženama. Većina tumora dojke ima značajan broj receptora za estrogen, progesteron i humani epidermalni faktor rasta 2 (HER2) te su klasificirani kao trostruko-pozitivni, odnosno trostruko-negativni. Liječenje može biti lokalno i sistemsko ili najčešće njihovom kombinacijom. Stanična linija je skup stanica, odnosno trajno uspostavljena stanična kultura koja se uzgaja u odgovarajućem mediju u kojem će neograničeno proliferirati pri kontroliranim uvjetima. U ovom diplomskom radu korištene su dvije stanične linije raka dojke, MDA-MB-231 i MCF-7 te biciklički seskviterpen nootkaton. Njegovo antiproliferativno djelovanje na dvije stanične linije raka ispitano je u prethodnom istraživanju korištenjem MTT-testa kojim je dokazano da nootkaton ima dobar sveukupni antiproliferativni učinak. Metabolomika je proučavanje svih metabolita u stanici, tkivu ili organizmu. To uključuje identifikaciju i kvantifikaciju staničnih metabolita. Ovdje je upotrijebljen postupak koji se temelji na gašenju metabolizma stanica te sakupljanju i ekstrakciji metabolita. Primijenjen je na dvije stanične linije raka dojke, MDA-MB-231 i MCF-7 uz dodatak i bez dodatka nootkatona (kontrola). Uzeta je koncentracija nootkatona od 0,5 mmol/L pri kojoj je prethodnim korištenjem MTT-testa nootkaton uspješno ubio 50 % stanica. Nakon sakupljanja i ekstrakcije, metaboliti su analizirani GC-MS metodom koja im ujedno omogućava identifikaciju. Dobiveni su kromatogrami i tablice s određenim podatcima te su uspoređene koncentracije identificiranih metabolita u dvije stanične linije raka dojke i zaključeno je do kojih je metaboličkih promjena došlo.
Abstract (english)
Proliferation, differentitation and survival of individual cells in a multicellular organism is carefully regulated, while in cancer cells there is no such regulation, they grow and divide uncontrollably, spread throughout the body and interfere with the function of normal tissues and organs. Cancer is the leading cause of death in the world. The most important thing in cancer pathology is to distinguish between benign and malignant tumors. Another way of classification is according to the type of cells from which the cancer is composed, according to which the main subtypes are carcinomas, sarcomas, lymphomas, leukemias and myelomas. Breast cancer is the leading cause of cancer-related deaths among women. Most breast tumors have a significant number of receptors for estrogen, progesterone and human epidermal growth factor 2 (HER2) and are classified as triple-positive or triple-negative. Treatment can be local or systemic or most often a combination of them. Cell line is a set of cells that is permanently established cell culture grown in a suitable medium in which it will proliferate indefinitely under controlled conditions. Two breast cancer cell lines, MDA-MB-231 and MCF-7, and the bicyclic sesquiterpene nootkatone were used in this diploma thesis. Its antiproliferative activity on two cancer cell lines was investigated in previous research using the MTT-assay, which proved that nootkatone has a good overall antiproliferative effect. Metabolomics is the study of all metabolites in a cell, tissue or organism. This includes the identification and quantification of cellular metabolites. Here, a procedure based on quenching of cells, harvesting and extraction of metabolites was used. It was applied on two breast cancer cell lines, MDA-MB-231 and MCF-7 with and without the addition of nootkatone (control). Nootkatone concentration of 0,5 mmol/L was taken, at which nootkatone successfully killed 50 % of the cells by previous use of the MTT-assay. After harvesting and extraction, the metabolites were analyzed by the GC-MS method, which also enables their identification. Chromatograms and tables with specific data were obtained, the concentrations of identified metabolites in two breast cancer cell lines were compared and it is concluded which metabolic changes occured.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:309494
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-11-17
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Created on2022-11-16 11:45:53