prikaz prve stranice dokumenta Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke
Embargo period 2025-03-31
master's thesis
Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2022. urn:nbn:hr:167:102828
Meić, Luka
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Institutional repository: Repository of the Faculty of chemistry and technology

Cite this document

Meić, L. (2022). Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:102828

Meić, Luka. "Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:102828

Meić, Luka. "Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:102828

Meić, L. (2022). 'Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 18 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:102828

Meić L. Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2022 [cited 2024 December 18] Available at: https://urn.nsk.hr/urn:nbn:hr:167:102828

L. Meić, "Metabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:167:102828

Metadata
TitleMetabolička analiza utjecaja [2, 3-b] piridinskog spoja na MDA-MB-231 i MCF-7 stanične linije raka dojke : diplomski rad
Title (english)Metabolomil analysis of impact of thieno [2, 3-b] pyridine compound on MDA-MB-231 and MCF-7 breast cancer cells : diploma thesis
AuthorLuka Meić
MentorMila Radan (mentor)
Committee memberAnte Prkić (predsjednik povjerenstva)
Committee memberMarina Tranfić Bakić (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2022-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Pojam metabolomika se odnosi na sustavnu identifikaciju i kvantifikaciju svih metabolita u određenom organizmu ili biološkom uzorku. Trenutno se koriste dva komplementarna pristupa za metabolička istraživanja: metaboličko profiliranje i metabolički otisak prsta (eng. fingerprint). Za metabolomičke analize koriste se tehnike za separaciju i tehnike visoke rezolucije za identifikaciju metabolita. Nakon što su analitičkim tehnikama dobiveni sirovi spektri, prije same statističke analize podataka, potrebna je predobrada neobrađenih podataka. Statistička analiza može biti univarijatna ili multivarijatna. Cilj ovog rada je istražiti utjecaj spoja (E)-3-amino-5-(3-(3-bromfenil)akriloil)-N-(3-kloro-2-metilfenil)-6- metiltieno[2,3-b]piridin-2-karboksamida (u nastavku teksta tieno[2,3-b]piridin) na metabolizam dvije vrste stanica raka dojke MCF-7 i MDA-MB-231. Za definiranje pikova, poravnanje RT vremena te označavanje i integraciju pikova korišten je softver Agilent MassHunter Qualitative Analysis. Platforma za analizu metabolomičkih podataka MetaboAnalyst je korištena za analizu različito eksprimiranih metabolita kroz dvije stanične linije. Rezultati analize pokazuju da tretman stanica tieno[2,3-b]piridinom ima veliki učinak na metabolizam glukoze odnosno energetske cikluse u stanici, a posebno na glikolizu, glukoneogenezu, metabolizam piruvata, pojavu Warburgova efekta i metabolizam inozitola. Tieno[2,3-b]piridin ima veću citotoksičnost za MDA-MB-231 u odnosu na MCF-7 staničnu liniju.
Abstract (english)
The term metabolomics refers to the systematic identification and quantification of all metabolites in certain organisms or biological samples. Currently, two complementary approaches are used in metabolic research: metabolic profiling and metabolic fingerprinting. Separation and high-resolution techniques for metabolite identification have been used in metabolomic analyses. After the raw spectra have been obtained using analytical techniques, preprocessing of the raw data is required before statistical analysis of the data. Statistical analyses can be univariate or multivariate. The aim of this study was to investigate the influence of compound (E)-3-amino-5-(3-(3- bromophenyl)acryloyl)-N-(3-chloro-2-methylphenyl)-6-methylthieno[2,3-b] pyridine-2-carboxamide (hereinafter thieno[2,3-b]pyridine) on the metabolism of two types of breast cancer cells MCF-7 and MDA- MB-231. Agilent MassHunter Qualitative Analysis software was used for peak definition, RT time alignment, and peak annotation and integration. MetaboAnalyst, a platform dedicated to metabolomic data analysis, was used to analyze differentially expressed metabolites across the two cell lines. The results of the analysis showed that the treatment of cells with thieno[2,3-b]pyridine had a great impact on glucose metabolism, that is, energy cycles in the cell, especially on glycolysis, gluconeogenesis, pyruvate metabolism, the occurrence of Warburg effect, and inositol metabolism. Thieno[2,3-b]pyridine showed greater cytotoxicity in MDA-MB-231 cells than in MCF-7 cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:102828
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-03-31
Terms of useLicence In copyright icon
Created on2023-03-31 09:06:41