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master's thesis
Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2017. urn:nbn:hr:167:821942
Župić, Mirna
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Institutional repository: Repository of the Faculty of chemistry and technology

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Župić, M. (2017). Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:821942

Župić, Mirna. "Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:821942

Župić, Mirna. "Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:821942

Župić, M. (2017). 'Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:821942

Župić M. Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:167:821942

M. Župić, "Citotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:821942

Metadata
TitleCitotoksično djelovanje izotiocijanata iz porodice kupusnjača na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad
Title (english)The cytotoxic effect og the Brassicaceae family isothiocyanates on various cell lines of human cancers measured by the MTT assay : diploma thesis
AuthorMirna Župić
MentorVedrana Čikeš Čulić (mentor)
Committee memberJanoš Terzić (predsjednik povjerenstva)
Committee memberIvana Mudnić (član povjerenstva)
Committee memberVedrana Čikeš Čulić (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Cilj istraživanja je bio ispitati citotoksični učinak određenih izotiocijanata iz porodice kupusnjača na dvije stanične linije humanih karcinoma: T24 i A1235. Korišteni izotiocijanati su: alil-, fenil-, benzil-, 2-fenil etil-, izopropil-, 4-metoksifenil-, 3-metoksifenil-, p-tolil- i 4-(metiltio)-fenil izotiocijanat. Stanice karcinoma mokraćnog mjehura tretirane su izotiocijanatima u koncentraciji od 5μM, 10μM, 0,5mM i 5mM, a stanice glioblastoma koncentracijama od 20 i 10μM. Citotoksičnost se određivala nakon 4, 24, 48 i 72h korištenjem MTT metode. Omjer apsorbancije stanica tretiranih vodenim otopinama izotiocijanata te apsorbancije onih koje nisu tretirane pokazatelj nam je citotoksične aktivnosti korištenih izotiocijanata.Najbolji citotoksični učinak na staničnu liniju T24 pokazao je izopropil izotiocijanat pri koncentraciji od 5mM nakon 72h inkubacije. Najbolji citotoksični učinak na staničnu liniju A1235 pokazao je benzil izotiocijanat pri koncentraciji od 20μM nakon 24h inkubacije.Ispitivani izotiocijanati pokazuju citotoksični učinak ovisan o vremenu inkubacije i koncentraciji. Djelovanje izotiocijanata nije uvijek razmjerno povećanju koncentracije i vremenu inkubacije, te u pojedinim slučajevima dolazi do oporavka stanica.Citotoksični učinak izotiocijanata, što je ujedno i hipoteza našeg ispitivanja, je potvrđen, a idući korak je potvrđivanje tih učinaka in vivo ispitivanjem na modelima karcinoma mokraćnog mjehura i glioblastoma kod životinja.
Abstract (english)
The aim of this research was to examine cytotoxic effects of certain isothiocyanates from the Brassicaceae family of plants on two cell lines of human carcinomas, T24 and A1235. The used isothiocyanates were: allyl-, phenyl-, benzyl-, 2- phenyl, ethyl-, isopropyl-, 4-methoxyphenyl, 3-methoxyphenyl, p-tolyl and 4- (methylthio)phenyl. Urinary bladder cancer cells were treated with isothiocyanates in the concentrations of 5μM, 10μM, 0.5mM and 5mM, while the glioblastoma cancer cells were treated with the 20 and 10μM concentrations. Cytotoxicity was established after the periods of 4, 24, 48 and 72 hours, using the MTT assay. The ratio of absorbance of cells treated with isothiocyanates in aqueous solutions and the ones not treated showed the cytotoxic activity of used isothiocyanates. The best cytotoxic effect on the cell line T24 was achieved by isopropyl isothiocyanate with the concentration of 5 mM after 72-hour incubation. The best cytotoxic effect on the cell line A1235 was achieved by benzyl isothiocyanate with the concentration of 20 μM after 24-hour incubation. Isothiocyanates tested showed cytotoxic effects dependent on incubation time and concentration. The effectiveness of isothiocyanates was not always correspondent to the increase of concentration and incubation time and in some cases cell recovery occured. The cytotoxic effect of isothiocyanates is confirmed, which was the hypothesis of the research, and the next step is the analysisof these effects by in vivo studies on the models of animal urinary bladder and glioblastoma cancers.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:821942
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2018-07-26 11:44:24