prikaz prve stranice dokumenta Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor
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master's thesis
Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2017. urn:nbn:hr:167:115672
Pauk, Renata
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Institutional repository: Repository of the Faculty of chemistry and technology

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Pauk, R. (2017). Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:115672

Pauk, Renata. "Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:115672

Pauk, Renata. "Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:115672

Pauk, R. (2017). 'Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:115672

Pauk R. Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:167:115672

R. Pauk, "Razvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:115672

Metadata
TitleRazvoj i vrednovanje kinetičke metode određivanja cisteina slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad
Title (english)Development and validation of the kinetic method for the determination of cysteine using sequential injection analysis with spectrophotometric detector : diploma thesis
AuthorRenata Pauk
MentorLea Kukoč Modun (mentor)
Committee memberOlivera Politeo (predsjednik povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
Committee memberLea Kukoč Modun (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Primjenom slijedne analize injektiranjem uz spektrofotometrijski detektor razvijena je i vrednovana kinetička metoda određivanja cisteina. Kinetička metoda se temelji na redoks reakciji gdje cistein kao reducens, reducira zeleni bakar(II) neokuproin kompleks do bakar(I) neokuproin kompleksa. Formirani ţuto- narančasti bakar(I) neokuproin kompleks pokazuje apsorpcijski maksimum pri valnoj duljini od 458 nm. Optimalni parametri protočnog sustava određeni su univarijantnom metodom te je temeljem toga konstruirana krivulja umjeravanja. Linearnost je postignuta u području koncentracija cisteina od 6,0 × 10 −7 do 8,0 × 10 −5 mol L −1 uz jednadţbu pravca: y = 3690,4 x + 0,0019 i korelacijski koeficijent R 2 = 0,9997, te su određene granica dokazivanja od 1,8 × 10 −7 mol L −1 i granica određivanja od 6,0 × 10 −7 mol L −1 . Provjeren je i utjecaj pratećih tvari koje se uobičajeno nalaze u sastavu farmaceutskih pripravaka te nije zabiljeţen njihov interferirajući učinak na određivanje analita. Vrednovanje metode provedeno je ispitivanjem ponovljivosti signala uz relativno standardno odstupanje od 3,35 % te zadovoljavajuće iskoristivosti, odnosno točnosti metode.
Abstract (english)
Using the sequential injection analysis with the spectrophotometric detector, the kinetic method for the determination of cysteine was developed and validated. The kinetic method is based on redox reaction where cysteine as a reductant, reduces green copper(II) neocuproine complex to copper(I) neocuproine complex. The formated yellow-orange copper(I) neocuproine complex shows an absorption maximum at wavelength of 458 nm. The optimum parameters of the flow system were determined by a univariate method and as a result the calibration curve was constructed. Linearity was achieved for the concentration range from 6.0  10 7 mol L 1 to 8.0  10 5 mol L 1 and it was described with the equation y = 3690.4 x + 0.0019 and correlation coefficient R 2 = 0.9997. The calculated limit of detection is 1.8  10 -7 mol L -1 and the limit of quantification is 6.0  10 7 mol L 1 . The influence of foreign substances commonly found in the composition of the pharmaceutical preparations was also examined, and no interfering effect on the analyte was noted. Validation of the method was performed by examining the repeatability of the signal with a relative standard deviation of 3.35%, and satisfactory recovery of method, or accuracy of the method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:115672
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2018-08-22 11:23:32