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master's thesis
Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2018. urn:nbn:hr:167:795821
Glumac, Mateo
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Institutional repository: Repository of the Faculty of chemistry and technology

Cite this document

Glumac, M. (2018). Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:795821

Glumac, Mateo. "Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:167:795821

Glumac, Mateo. "Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:167:795821

Glumac, M. (2018). 'Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:795821

Glumac M. Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2018 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:167:795821

M. Glumac, "Određivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:167:795821

Metadata
TitleOdređivanje utjecaja farnezola i nerolidola na p38 MAPK stanični put : diplomski rad
Title (english)Determination of farnesol and nerolidol activity on p38 MAPK cell signaling pathway : diploma thesis
AuthorMateo Glumac
MentorMila Radan (mentor)
MBZ: 3119068
Committee memberMladen Miloš (predsjednik povjerenstva)
Committee memberAni Radonić (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2018-10-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Interdisciplinary Natural Sciences
Abstract
Koristeći se western blot tehnikom kao metodom za identifikaciju i kvantifikaciju proteina određivan je utjecaj farnezola i nerolidola na ekspresiju p38 MAP kinaza kod tri stanične linije raka: raka mokraćnog mjehura(T24, TCC SUP) i glioblastoma (LN229). p38 MAP kinaze ključni su dio p38 MAPK staničnog signalnog puta koji je zadužen za transkripciju, popravak oštećenja na stanici i borbu protiv kancerogeneze. Na sve tri stanične linije ispitivan je utjecaj seskviterpenskih alkohola (farnezola i nerolidola) na ekspresiju p38 MAP kinaza te je rezultat uspoređen s kontrolnim uzorcima čime je ostvarena objektivna usporedba aktivnosti ispitivanih spojeva. Iz dobivenih eksperimentalnih podataka može se zaključiti da konstitucijski izomeri farnezol i nerolidol različito utječu na ispitivani signalni put u različitim staničnim linijama. Farnezol pokazuje slaba inhibitorska svojstva za T24 i TCC SUP staničnu liniju dok kod LN229 pokazuje snažnu aktivaciju signalnog puta. Nerolidol pokazuje snažnu inhibiciju kod T24 i LN229 stanične linije dok kod linije TCC SUP pokazuje aktivacijsku sposobnost. Dobiveni rezultati sugeriraju na različito vezivanje farnezola i nerolidola na stanične receptore kod istih i različitih staničnih linija što je veoma zanimljiv rezultat za daljnja istraživanja. Detaljan opis eksperimentalnog rada prikazan je u radu zajedno s rezultatima eksperimenta.
Abstract (english)
Using the Western blot technique as a method for identification and quantification of proteins p38 MAP kinaze quantity was determined in three cancer cell lines: urinary bladder cancer (T24, TCC SUP) and glioblastoma (LN229). p38 MAPKs are key molecules in the p38 MAPK cell signaling pathway which is in charge of transcription, damage repairs and anti-cancer activity. All cell lines were tested for sesquiterpene alcohol (farnesol and nerolidol) activity on p38 MAPK quantity and the results was compared with control samples to gain an objective conclusion on their activity. Experimental data shows that constitutional isomers farnesol and nerolidol show different effect on tested pathway in different cell lines. Farnesol shows low inhibitory activity for T24 and TCC SUP but it shows a high activation ability for LN229 cell line. Nerolidol shows a high inhibitory activity for T24 and LN229 but it shows activation for TCC SUP cell line. Experimental data suggest that farnesol and nerolidol bind differently on the cell receptors within the same cell line and on the different cell lines making the results of this thesis interesting topic for future research. Detailed description of the experiment is shown in the thesis as well as experimental results data.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:795821
ProjectNumber: IP-2014-09-6897
Title: Istraživanje bioaktivnih spojeva iz dalmatinskogbilja: njihov antioksidacijski karakter i utjecaj na enzimsku inhibiciju i zdravlje
Acronym: BioActCom
Leader: Mladen Miloš
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2019-03-06 11:55:43