prikaz prve stranice dokumenta Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.
  Download
PDF 6.2 MB
Scientific paper - Original scientific paper
Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.
Journal of virology, 87 (2013), 12; 6943-6954. https://doi.org/10.1128/JVI.00407-13
Roviš, Tihana Lenac; Bailer, Susanne M.; Pothineni, Venkata R.; Ouwendijk, Werner J. D.; Šimic, Hrvoje; Babić, Marina; Miklić, Karmela; Malić, Suzana; Verweij, Marieke C.; Baiker, Armin; Gonzalez, Orland; von Brunn, Albrecht; Zimmer, Ralf; Frueh, Klaus; Verjans, Georges M. G. M.; Jonjić, Stipan; Haas, Jürgen More authors...

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Roviš, T. L., Bailer, S. M., Pothineni, V. R., Ouwendijk, W. J. D., Šimic, H., Babić, M. ... Haas, J. (2013). Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.. Journal of virology, 87. (12), 6943-6954. doi: 10.1128/JVI.00407-13

Roviš, Tihana Lenac, et al. "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.." Journal of virology, vol. 87, no. 12, 2013, pp. 6943-6954. https://doi.org/10.1128/JVI.00407-13

Roviš, Tihana Lenac, Susanne M. Bailer, Venkata R. Pothineni, Werner J. D. Ouwendijk, Hrvoje Šimic, Marina Babić, Karmela Miklić, et al. "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.." Journal of virology 87, no. 12 (2013): 6943-6954. https://doi.org/10.1128/JVI.00407-13

Roviš, T. L., et al. (2013) 'Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.', Journal of virology, 87(12), pp. 6943-6954. doi: 10.1128/JVI.00407-13

Roviš TL, Bailer SM, Pothineni VR, Ouwendijk WJD, Šimic H, Babić M, and sur.. Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.. Journal of virology [Internet]. 2013 [cited 2024 November 09];87(12):6943-6954. doi: 10.1128/JVI.00407-13

T. L. Roviš, et al., "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.", Journal of virology, vol. 87, no. 12, pp. 6943-6954, 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:359337. [Accessed: 09 November 2024]

Metadata
Title (english)Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.
AuthorTihana Lenac Roviš
AuthorSusanne M. Bailer
AuthorVenkata R. Pothineni
AuthorWerner J. D. Ouwendijk
AuthorHrvoje Šimic
AuthorMarina Babić
AuthorKarmela Miklić
AuthorSuzana Malić
AuthorMarieke C. Verweij
AuthorArmin Baiker
AuthorOrland Gonzalez
AuthorAlbrecht von Brunn
AuthorRalf Zimmer
AuthorKlaus Frueh
AuthorGeorges M. G. M. Verjans
AuthorStipan Jonjić
AuthorJürgen Haas
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of virology
Numberingvol. 87, no. 12, pp. 6943-6954
e-ISSN1098-5514
DOIhttps://doi.org/10.1128/JVI.00407-13
URN:NBNurn:nbn:hr:184:359337
Publication2013
ProjectNumber: 229585
Title (english): The Center for Antibody Production Rijeka: Upgrading the Central Research and Service Infrastructure for the South Eastern Region of Europe
Acronym: CAPRI2010
Jurisdiction: eu
Funding stream: FP7
Document URLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676123/
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-06-21 07:18:58