prikaz prve stranice dokumenta Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection
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Scientific paper - Original scientific paper
Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection
Periodicum biologorum, 112 (2010), 1; 105-109. urn:nbn:hr:184:399913
Vasiljev Marchesi, Vanja; Frančišković, Ivica; Bastiančić, Luka; Rukavina, Tomislav

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Vasiljev Marchesi, V., Frančišković, I., Bastiančić, L. & Rukavina, T. (2010). Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection. Periodicum biologorum, 112. (1), 105-109. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:399913

Vasiljev Marchesi, Vanja, et al. "Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection." Periodicum biologorum, vol. 112, no. 1, 2010, pp. 105-109. https://urn.nsk.hr/urn:nbn:hr:184:399913

Vasiljev Marchesi, Vanja, Ivica Frančišković, Luka Bastiančić and Tomislav Rukavina. "Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection." Periodicum biologorum 112, no. 1 (2010): 105-109. https://urn.nsk.hr/urn:nbn:hr:184:399913

Vasiljev Marchesi, V., et al. (2010) 'Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection', Periodicum biologorum, 112(1), pp. 105-109. Available at: https://urn.nsk.hr/urn:nbn:hr:184:399913 (Accessed 14 March 2025)

Vasiljev Marchesi V, Frančišković I, Bastiančić L, Rukavina T. Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection. Periodicum biologorum [Internet]. 2010 March 31 [cited 2025 March 14];112(1):105-109. Available at: https://urn.nsk.hr/urn:nbn:hr:184:399913

V. Vasiljev Marchesi, I. Frančišković, L. Bastiančić and T. Rukavina, "Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection", Periodicum biologorum, vol. 112, no. 1, pp. 105-109, March 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:399913. [Accessed: 14 March 2025]

Metadata
Title (english)Combined effect of monoclonal antilipopolysaccharide antibody and ceftazidime in intranasal mice model of Klebsiella pneumoniae infection
AuthorVanja Vasiljev Marchesi
AuthorIvica Frančišković
AuthorLuka Bastiančić
AuthorTomislav Rukavina
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Social Medicine and Epidemiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Background and Purpose: The evolution of microorganisms in the sense of antimicrobial resistance is an emerging problem, especially in hospital settings. It seriously complicates the ability to combat severe infections. Bacteria of the genus Klebsiella are well known bacterial pathogens that cause a wide variety of infections. Numerous recent articles have reported the growing trend of antimicrobial resistance among clinical isolates of Klebsiella. Besides capsular antigen the most important virulence factor of genus Klebsiella is lipopolysaccharide (LPS). Therefore, previously generated antilipopolysaccharide monoclonal antibody for O1 antigen of Klebsiella pneumoniae was used in this study. The purpose of this study was to examine the existence of synergy between antilipopolysaccharide monoclonal antibody Ru-O1 (mAb Ru-O1) and ceftazidime in a model of lung Klebsiella infection. Materials and Methods: The study was conducted using Klebsiella pneumoniae strain Caroli (O1:K2) to inoculate BALB/c mice by intranasal route. Mice were lethally infected with the bacteria. The effects of mAb Ru-O1 and ceftazidime, as a single treatment protocol or in combination, were monitored on the survival of experimental animals. Results: The overall survival rates in groups pretreated only with mAb Ru-O1 prior to infection or treated only with ceftazidime 24 hours after infectionwere 33%. The outcome of infection was best in a group of mice that received both mAb and single dose of ceftazidime with overall survival rate of 66%. Conclusions: The study shows that the combination treatment with anti-LPS mAb Ru-O1 and ceftazidime in amice model of lethal Klebsiella pneumoniae pneumonia exerts synergistic effect and enhances the survival of experimental animals compared to animals treated with mAb Ru-O1 or ceftazidime alone.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titlePeriodicum biologorum
Numberingvol. 112, no. 1, pp. 105-109
p-ISSN0031-5362
e-ISSN1849-0964
URN:NBNurn:nbn:hr:184:399913
Publication2010-03-31
Document URLhttp://hrcak.srce.hr/52732
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-08-11 08:36:51