| Abstract | Enterococcus faecalis bakterija je kojoj se nedavno počela pridavati pažnja zbog sve učestalijih što hospitalnih što drugih infekcija. Prirodno se nalaze u crijevima čovjeka i životinja. No kada dospiju na mjesto gdje nisu dio prirodne flore mogu izazavati upale. Najčešće govorimo o mokraćnom sustavu gdje kao posljedicu rasta i razmnožavanja uzrokuju urinoinfekt. u ovom smo radu ispitali čimbenike virulencije te osjetljivost na antibiotike enterokoka izoliranih iz urina.
Od čimbenika virulencije ispitivani su enzimi proteaza, lipaza, želatinaza, katalaza; ispitivana je hidroliza eskulina, hemoliza, rezistencija na lizozim, pokretljivost, izvanstanična sluz i biofilm, te agregacija i osjetljivost na antibiotike. Nakon provedenog istraživanja dokazano je da su svi sojevi katalaza negativni, dok su svi eskulin pozitivni. U više od 80% izolata dokazana je γ-hemoliza. Od ostalih enzima proteazu producira 59% izolata, lipazu 95% i želatinazu 70%. Tendenciju stvaranja biofilma i izvanstanične sluzi posjeduje 73% bakterija. Na koncentraciju od 60 mg/ml lizozima osjetljivo je 35% bakterija, dok su na niže koncentracije lizozima (20, 40 mg/ml lizozima) svi izolati rezistentni. Za agregaciju možemo reći da 31% bakterija agregira u postotku većem od 90%. Od antibiotika važno je izdvojiti gentamicin za kojeg postoji rezistencija kod 23% izolata te na norfloksacin 20% izolata.
E. faecalis posjeduju više čimbenika virulencije, između kojih bi istaknula produkciju izvanstanične sluzi i biofilma, te njihovu sposobnost razgradnje kolagena u tijelu pomoću enzima želatinaze |
| Abstract (english) | Enterococcus faecalis bacterium has recently begun to pay attention due to the more frequent hospital infection and other infections. They are naturally found in the intestinal flora of man and animal. But when they reach a place where they are not part of a natural flora can cause inflammation. Most commonly, we are talking about urinary system, which as a result of growth and reproduction cause urine infestation. In this paper, we investigated virulence factors and sensitivity to the antibiotics of enterococci isolated from urine.
Virulence factors who were investigated are protease enzymes, lipases, gelatinases, catalases; hemolysis, lysozyme resistance, mobility, extracellular mucus and biofilm, aggregation and sensitivity to antibiotics. After the study has been shown, all catalase strains are negative, while they are all esculin positive. In more than 80% of isolates, γ-hemolysis has been proven. Of the other enzymes protease is produced by 59% isolates, 95% lipase and 70% gelatinase. The tendency to create biofilm and extracellular mucus has 73% bacteria. At concentrations of 60 mg / ml lysozyme, 35% bacteria are susceptible, while all isolates with lysozyme lower concetration (20, 40 mg/ml lysozyme) are resistant. For aggregation we can say that 31% of bacteria aggregate in percentage more than 90%. Of the antibiotics it is important to distinguish gentamicin with resistance to 23% of isolates and norfloxacin with 20% isolates.
E. faecalis possesses several virulence factors, among which the most important are extracellular mucus production and biofilms production, and their ability to degrade collagen in the body by the gelatin enzyme |
