Staphylococci are gram pozitiv bacteria, they are usually part of the normal flora, especially skin and mucose membrans, so they cause infection only by a weakened immune system. Most medically important are Staphylococcus aureus and Staphylococcus epidermidis. These bacteria show resistance to the most common antibiotics so they are a major problem in treatment od staphylococcal infection. Therfore in this work antibacterial effect of six essential oils (juniper, sage, lavander, immortellje autumn and spring, thuja) on two difrent spicies of Staphylococcus, S. aureus and S. epidermidis was tested. To examine whether the resistant strains of tested species show sensitivity to the herb essential oils, methicillin resistant S. aureus and methicillin resistant S. epidermidis were used. In the research of antimicrobial activity three methods were used: diffusion , dilution and test the effect of the volatile components of the herb essential oils. Results show anitmicrobial activity of lavander essential oil on resistant strains MRSA and MRSE, and immortelle autumn essential oil on S. aureus regardless of the resistance. This study demonstrates the potential use of essential oils as antibacterial agents for the treatment of staphylococcal infections, including infections caused by methicillin resistant strains.