prikaz prve stranice dokumenta MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
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Scientific paper - Original scientific paper
MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
Scientific Reports, 8 (2018); 13688. https://doi.org/10.1038/s41598-018-31844-1
Zaytseva, Olga O.; Jansen, Bas C.; Hinić, Maja; Mrčela, Mia; Genadij, Razdorov; Stojković, Ranko; Erhardt, Julija; Brizić, Ilija; Jonjić, Stipan; Pezer, Marija; Lauc, Gordan

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Zaytseva, O. O., Jansen, B. C., Hinić, M., Mrčela, M., Genadij, R., Stojković, R. ... Lauc, G. (2018). MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS. Scientific Reports, 8.. doi: 10.1038/s41598-018-31844-1

Zaytseva, Olga O., et al. "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS." Scientific Reports, vol. 8, 2018. https://doi.org/10.1038/s41598-018-31844-1

Zaytseva, Olga O., Bas C. Jansen, Maja Hinić, Mia Mrčela, Razdorov Genadij, Ranko Stojković, Julija Erhardt, et al. "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS." Scientific Reports 8 (2018). https://doi.org/10.1038/s41598-018-31844-1

Zaytseva, O. O., et al. (2018) 'MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS', Scientific Reports, 8. doi: 10.1038/s41598-018-31844-1

Zaytseva OO, Jansen BC, Hinić M, Mrčela M, Genadij R, Stojković R, and sur.. MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS. Scientific Reports [Internet]. 2018 August 12 [cited 2024 November 23];8. doi: 10.1038/s41598-018-31844-1

O. O. Zaytseva, et al., "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS", Scientific Reports, vol. 8, August 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:098355. [Accessed: 23 November 2024]

Metadata
Title (english)MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
AuthorOlga O. Zaytseva
AuthorBas C. Jansen
AuthorMaja Hinić
AuthorMia Mrčela
AuthorRazdorov Genadij
AuthorRanko Stojković
AuthorJulija Erhardt
AuthorIlija Brizić
AuthorStipan Jonjić
AuthorMarija Pezer
AuthorGordan Lauc
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract (english)
Immunoglobulin G (IgG) N-glycosylation is crucial for its effector functions. It is a complex trait, and large sample sets are needed to discover multiple genetic factors that underlie it. While in humans such high-throughput studies of IgG N-glycans became usual, only one has been carried out in mice. Here we describe and validate a method for the relative quantification of IgG Fc-linked N-glycans in a subclass- specific manner using nano-reverse phase liquid chromatography coupled with mass-spectrometry (nanoRP-LC-MS) applied to murine IgG. High-throughput data processing is ensured by the LaCyTools software. We have shown that IgG isolation procedure is the main source of technical variation in the current protocol. The major glycoforms were quantified reliably with coefficients of variation below 6% for all the analytes with relative abundances above 5%. We have applied our method to a sample set of 3 inbred strains: BALB/c, C57BL/6 and C3H and observed differences in subclass-specific and strain - specific N - glycosylation of IgG, suggesting a significant genetic component in the regulation of Fc- linked IgG N - glycosylation
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 8, 13688
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-018-31844-1
URN:NBNurn:nbn:hr:184:098355
Publication2018-08-12
Document URLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135756/pdf/41598_2018_Article_31844.pdf
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-09-29 11:38:22