prikaz prve stranice dokumenta CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors
  Download
PDF 753.15 KB
Scientific paper - Original scientific paper
CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors
American Journal of Transplantation, 12 (2012), 6; 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x
Poglitsch, M.; Weichhart, T.; Hecking, M.; Werzowa, J.; Katholnig, K.; Antlanger, M.; Krmpotić, Astrid; Jonjić, Stipan; Hörl, W. H.; Zlabinger, G. J.; Puchhammer, E.; Säemann, M. D.

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Poglitsch, M., Weichhart, T., Hecking, M., Werzowa, J., Katholnig, K., Antlanger, M. ... Säemann, M. D. (2012). CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors. American Journal of Transplantation, 12. (6), 1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., et al. "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors." American Journal of Transplantation, vol. 12, no. 6, 2012, pp. 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., T. Weichhart, M. Hecking, J. Werzowa, K. Katholnig, M. Antlanger, Astrid Krmpotić, et al. "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors." American Journal of Transplantation 12, no. 6 (2012): 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., et al. (2012) 'CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors', American Journal of Transplantation, 12(6), pp. 1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

Poglitsch M, Weichhart T, Hecking M, Werzowa J, Katholnig K, Antlanger M, and sur.. CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors. American Journal of Transplantation [Internet]. 2012 June [cited 2024 December 24];12(6):1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

M. Poglitsch, et al., "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors", American Journal of Transplantation, vol. 12, no. 6, pp. 1458-1468, June 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:984654. [Accessed: 24 December 2024]

Metadata
Title (english)CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors
AuthorM. Poglitsch
AuthorT. Weichhart
AuthorM. Hecking
AuthorJ. Werzowa
AuthorK. Katholnig
AuthorM. Antlanger
AuthorAstrid Krmpotić
AuthorStipan Jonjić
AuthorW. H. Hörl
AuthorG. J. Zlabinger
AuthorE. Puchhammer
AuthorM. D. Säemann
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Human cytomegalovirus (CMV) remains one of the most important pathogens following solid-organ transplantation. Mounting evidence indicates that mammalian target of rapamycin (mTOR) inhibitors may decrease the incidence of CMV infection in solid- organ recipients. Here we aimed at elucidating the molecular mechanisms of this effect by employing a human CMV (HCMV) infection model in human macrophages, since myeloid cells are the principal in vivo targets of HCMV. We demonstrate a highly di- vergent host cell permissiveness for HCMV with opti- mal infection susceptibility in M2 but not M1 polarized macrophages. Employing an ultrahigh purified HCMV stock we observed rapamycin-independent viral entry and induction of IFN-b transcripts, but no proinflam- matory cytokines or mitogen-activated protein kinases and mTOR activation early after infection. However, in the late infection phase, sustained mTOR activa- tion was observed in HCMV-infected cells and was required for efficient viral protein synthesis including the viral late phase proteins pUL-44 and pp65. Accord- ingly, rapamycin strongly suppressed CMV replication 3 and 5 days postinfection in macrophages. In conclu- sion, these data indicate that mTOR is essential for virus replication during late phases of the viral cycle in myeloid cells and might explain the potent anti-CMV effects of mTOR inhibitors after organ transplantation
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleAmerican Journal of Transplantation
Numberingvol. 12, no. 6, pp. 1458-1468
p-ISSN1600-6135
DOIhttps://doi.org/10.1111/j.1600-6143.2012.04002.x
URN:NBNurn:nbn:hr:184:984654
Publication2012-06
Publication2012-03-05
Document URLhttp://doi.wiley.com/10.1111/j.1600-6143.2012.04002.x
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-08 10:56:29