prikaz prve stranice dokumenta Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2
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Scientific paper - Original scientific paper
Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2
Journal of Virology, 85 (2011), 19; 10346-10353. https://doi.org/10.1128/JVI.00545-11
Jordan, Stefan; Krause, Johannes; Prager, Adrian; Mitrović, Maja; Jonjić, Stipan; Koszinowski, Ulrich H.; Adler, Barbara

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Jordan, S., Krause, J., Prager, A., Mitrović, M., Jonjić, S., Koszinowski, U. H. & Adler, B. (2011). Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2. Journal of Virology, 85. (19), 10346-10353. doi: 10.1128/JVI.00545-11

Jordan, Stefan, et al. "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2." Journal of Virology, vol. 85, no. 19, 2011, pp. 10346-10353. https://doi.org/10.1128/JVI.00545-11

Jordan, Stefan, Johannes Krause, Adrian Prager, Maja Mitrović, Stipan Jonjić, Ulrich H. Koszinowski and Barbara Adler. "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2." Journal of Virology 85, no. 19 (2011): 10346-10353. https://doi.org/10.1128/JVI.00545-11

Jordan, S., et al. (2011) 'Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2', Journal of Virology, 85(19), pp. 10346-10353. doi: 10.1128/JVI.00545-11

Jordan S, Krause J, Prager A, Mitrović M, Jonjić S, Koszinowski UH, and sur.. Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2. Journal of Virology [Internet]. 2011 August 03 [cited 2024 May 14];85(19):10346-10353. doi: 10.1128/JVI.00545-11

S. Jordan, et al., "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2", Journal of Virology, vol. 85, no. 19, pp. 10346-10353, August 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:976493. [Accessed: 14 May 2024]

Metadata
Title (english)Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2
AuthorStefan Jordan
AuthorJohannes Krause
AuthorAdrian Prager
AuthorMaja Mitrović
AuthorStipan Jonjić
AuthorUlrich H. Koszinowski
AuthorBarbara Adler
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Murine cytomegalovirus (MCMV) Smith strain has been cloned as a bacterial artificial chromosome (BAC) named pSM3fr and used for analysis of virus gene functions in vitro and in vivo. When sequencing the complete BAC genome, we identified a frameshift mutation within the open reading frame (ORF) encoding MCMV chemokine homologue MCK-2. This mutation would result in a truncated MCK-2 protein. When mice were infected with pSM3fr-derived virus, we observed reduced virus production in salivary glands, which could be reverted by repair of the frameshift mutation. When looking for the source of the mutation, we consistently found that virus stocks of cell culture-passaged MCMV Smith strain are mixtures of viruses with or without the MCK-2 mutation. We conclude that the MCK-2 mutation in the pSM3fr BAC is the result of clonal selection during the BAC cloning procedure.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Virology
Numberingvol. 85, no. 19, pp. 10346-10353
p-ISSN0022-538X
DOIhttps://doi.org/10.1128/JVI.00545-11
URN:NBNurn:nbn:hr:184:976493
Publication2011-08-03
Publication2011-10-01
ProjectNumber: 062-0621261-1263
Title (croatian): Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttp://jvi.asm.org/cgi/doi/10.1128/JVI.00545-11
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-09 08:27:51