prikaz prve stranice dokumenta Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity
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Scientific paper - Original scientific paper
Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity
Cell Discovery, 1 (2015), 1; 15036. https://doi.org/10.1038/celldisc.2015.36
Glasner, Ariella; Roth, Ziv; Varvak, Alexander; Miletić, Antonija; Isaacson, Batya; Bar-On, Yotam; Jonjić, Stipan; Khalaila, Isam; Mandelboim, Ofer

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Glasner, A., Roth, Z., Varvak, A., Miletić, A., Isaacson, B., Bar On, Y. ... Mandelboim, O. (2015). Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity. Cell Discovery, 1. (1). doi: 10.1038/celldisc.2015.36

Glasner, Ariella, et al. "Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity." Cell Discovery, vol. 1, no. 1, 2015. https://doi.org/10.1038/celldisc.2015.36

Glasner, Ariella, Ziv Roth, Alexander Varvak, Antonija Miletić, Batya Isaacson, Yotam Bar On, Stipan Jonjić, Isam Khalaila and Ofer Mandelboim. "Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity." Cell Discovery 1, no. 1 (2015). https://doi.org/10.1038/celldisc.2015.36

Glasner, A., et al. (2015) 'Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity', Cell Discovery, 1(1). doi: 10.1038/celldisc.2015.36

Glasner A, Roth Z, Varvak A, Miletić A, Isaacson B, Bar On Y, and sur.. Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity. Cell Discovery [Internet]. 2015 December 22 [cited 2024 November 26];1(1). doi: 10.1038/celldisc.2015.36

A. Glasner, et al., "Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity", Cell Discovery, vol. 1, no. 1, December 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:585910. [Accessed: 26 November 2024]

Metadata
Title (english)Identification of putative novel O-glycosylations in the NK killer receptor Ncr1 essential for its activity
AuthorAriella Glasner
AuthorZiv Roth
AuthorAlexander Varvak
AuthorAntonija Miletić
AuthorBatya Isaacson
AuthorYotam Bar-On
AuthorStipan Jonjić
AuthorIsam Khalaila
AuthorOfer Mandelboim
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Natural killer (NK) cells kill tumor and virus-infected cells using activating NK cell receptors. One of the major NK-activating receptors is NKp46 and its mouse ortholog Ncr1. NKp46/Ncr1 is expressed exclusively on NK cells and on a subset of innate lymphoid cells. NKp46/Ncr1 was shown to be involved in a myriad of pathologies and immunological settings. Specifically, NKp46/Ncr1 was shown to interact with the viral hemagglutinin (HA) protein and with an unknown tumor/cellular ligand. NKp46 and Ncr1 are structurally similar; however, they are substantially different in their glycosylation patterns. Although the human NKp46 carries both O- and N-glycosylations that are essential for its activity, the mouse Ncr1 was predicted to have N-linked glycosylations only. Here we discovered using prediction algorithms and high-performance liquid chromatography analysis that Ncr1 carries two putative novel O-glycosylations, one of which (Thr 225) is conserved in NKp46. We next used surface plasmon resonance, biochemical, mutational and functional in vitro and in vivo assays to demonstrate that the putative O-glycosylations of Ncr1 are critical for its function.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCell Discovery
Numberingvol. 1, no. 1, 15036
e-ISSN2056-5968
DOIhttps://doi.org/10.1038/celldisc.2015.36
URN:NBNurn:nbn:hr:184:585910
Publication2015-12-22
Publication2015-12
ProjectNumber: 322693
Title (english): Strengthening adaptive immunity via innate immunity: enhancing the CD8 T cell response by using the NKG2D ligand expressed in a herpesvirus vector
Acronym: STADVINN
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 320473
Title (english): Recognition of bacteria by NK cells
Acronym: BACNK
Jurisdiction: eu
Funding stream: FP7
Document URLhttp://www.nature.com/articles/celldisc201536
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-22 22:22:31