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master's thesis
UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM
Rijeka: University of Rijeka, Faculty of Medicine, 2018. urn:nbn:hr:184:143531
Nora, Valerija
University of Rijeka
Faculty of Medicine
Department of Microbiology and Parasitology

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Nora, V. (2018). UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:143531

Nora, Valerija. "UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:143531

Nora, Valerija. "UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM." Master's thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:143531

Nora, V. (2018). 'UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 29 April 2024, https://urn.nsk.hr/urn:nbn:hr:184:143531

Nora V. UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 April 29] Available at: https://urn.nsk.hr/urn:nbn:hr:184:143531

V. Nora, "UTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:143531

Metadata
TitleUTJECAJ INHIBITORA I INDUKTORA AUTOFAGIJE NA RAZMNOŽAVANJE BAKTERIJA LEGIONELLA PNEUMOPHILA I FRANCISELLA NOVICIDA U AMEBI DICTYOSTELIUM DISCOIDEUM
AuthorValerija Nora
MentorMarina Šantić (mentor)
Committee memberMarin Tota (predsjednik povjerenstva)
Committee memberJagoda Ravlić-Gulan (član povjerenstva)
Committee memberMarina Šantić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Rijeka
Defense date and country2018-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Autofagija ili autofagocitoza je evolucijski očuvan katabolički proces koji uključuje degradaciju citoplazmatskih komponenti. Tijekom infekcije različiti mikroroganizmi različito reguliraju proces autofagije. Istraživanja su pokazala da L. pneumophila i F. novicida u nekim stanicama potiču autofagiju, a u drugim suprimiraju proces autofagije u korist unutarstanične replikacije. Najveći broj istraživanja proveden je u stanicama sisavaca, a vrlo malo se zna o utjecaju autofagije prilikom infekcije stanica ameba s bakterijama Francisella i Legionella. Klorokin i vortmanin imaju ulogu da blokiraju autofagiju, dok rapamicin inhibiranjem mTOR receptora potiče autofagiju. Cilj ovog istraživanja bio je utvrditi utjecaj klorokina, rapamicina i vortmanina na razmnožavanje L. pneumophila i F. novicida unutar amebe Dictyostelium discoideum. Također, željeli smo utvrditi ovisi li utjecaj inhibitora i induktora autofagije o koncentraciji i vremenu dodavanja. Rezultati su pokazali kako dodatak inhibitora autofagije, klorokina i vortmanina, negativno utječe na razmnožavanje bakterija. Međutim, prilikom dodatka rapamicina, induktora autofagije, bakterije se bolje razmnožavaju unutar amebe. Također, iz toga se može zaključiti kako fomiranje autofagične vakuole pomaže bakterijama L. pneumophila i F. novicida prilikom preživljavanja i razmnožavanja unutar amebe.
Abstract (english)
Autophagy or autophagocytosis is an evolutionally preserved catabolic process that involves the degradation of cytoplasmic components. During infection, various microorganisms regulate the autophagy process differently. Studies have shown that L. pneumophila and F. novicida in some cells stimulate autophagy, and in others suppress the autophagy process in favor of intracellular replication. Most of the studies were performed in mammalian cells, and very little is known about the autophagy process in the amoeba cell after infection with Francisella and Legionella. Chloroquine and wortmannin plays a role in blocking the autophagy, while rapamycin in iducing the autophagy by inhibiting mTOR receptor. The aim of this study was to determine the influence of chloroquine, rapamycin and wortmannin on replication of L. pneumophila and F. novicida within Dyctiostelium discoideum. Also, we wanted to determine how depends the concentration and time of addition on influence of inhibitors and inductor of autophagy. The results have shown that treatment of cells with autophagy inhibitors, chloroquine and wortmannin has a negative effect on survival and replication of Legionella and Francisella. In contrast, induction of autophagy in amoebae cells results of high number of intracellular bacteria. We can conclude that the formation of an autophagic vacuole support the intracellular replication of L. pneumophila and F. novicida in Dictyostelium discoideum.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:143531
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-12-07 09:45:11