prikaz prve stranice dokumenta The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89
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Scientific paper - Original scientific paper
The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89
Journal of Virology, 87 (2013), 3; 1720-1732. https://doi.org/10.1128/JVI.01955-12
Borst, Eva Maria; Kleine-Albers, Jennifer; Gabaev, Ildar; Babić, Marina; Wagner, Karen; Binz, Anne; Degenhardt, Inga; Kalesse, Markus; Jonjić, Stipan; Bauerfeind, Rudolf; Messerle, Martin

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Borst, E. M., Kleine Albers, J., Gabaev, I., Babić, M., Wagner, K., Binz, A. ... Messerle, M. (2013). The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89. Journal of Virology, 87. (3), 1720-1732. doi: 10.1128/JVI.01955-12

Borst, Eva Maria, et al. "The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89." Journal of Virology, vol. 87, no. 3, 2013, pp. 1720-1732. https://doi.org/10.1128/JVI.01955-12

Borst, Eva Maria, Jennifer Kleine Albers, Ildar Gabaev, Marina Babić, Karen Wagner, Anne Binz, Inga Degenhardt, et al. "The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89." Journal of Virology 87, no. 3 (2013): 1720-1732. https://doi.org/10.1128/JVI.01955-12

Borst, E. M., et al. (2013) 'The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89', Journal of Virology, 87(3), pp. 1720-1732. doi: 10.1128/JVI.01955-12

Borst EM, Kleine Albers J, Gabaev I, Babić M, Wagner K, Binz A, and sur.. The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89. Journal of Virology [Internet]. 2013 February 01 [cited 2024 May 14];87(3):1720-1732. doi: 10.1128/JVI.01955-12

E. M. Borst, et al., "The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89", Journal of Virology, vol. 87, no. 3, pp. 1720-1732, February 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:589071. [Accessed: 14 May 2024]

Metadata
Title (english)The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89
AuthorEva Maria Borst
AuthorJennifer Kleine-Albers
AuthorIldar Gabaev
AuthorMarina Babić
AuthorKaren Wagner
AuthorAnne Binz
AuthorInga Degenhardt
AuthorMarkus Kalesse
AuthorStipan Jonjić
AuthorRudolf Bauerfeind
AuthorMartin Messerle
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
ABSTRACT Cleavage of human cytomegalovirus (HCMV) genomes as well as their packaging into capsids is an enzymatic process mediated by viral proteins and therefore a promising target for antiviral therapy. The HCMV proteins pUL56 and pUL89 form the terminase and play a central role in cleavage-packaging, but several additional viral proteins, including pUL51, had been suggested to contribute to this process, although they remain largely uncharacterized. To study the function of pUL51 in infected cells, we constructed HCMV mutants encoding epitope-tagged versions of pUL51 and used a conditionally replicating virus (HCMV-UL51-ddFKBP), in which pUL51 levels could be regulated by a synthetic ligand. In cells infected with HCMV-UL51-ddFKBP, viral DNA replication was not affected when pUL51 was knocked down. However, no unit-length genomes and no DNA-filled C capsids were found, indicating that cleavage of concatemeric HCMV DNA and genome packaging into capsids did not occur in the absence of pUL51. pUL51 was expressed mainly with late kinetics and was targeted to nuclear replication compartments, where it colocalized with pUL56 and pUL89. Upon pUL51 knockdown, pUL56 and pUL89 were no longer detectable in replication compartments, suggesting that pUL51 is needed for their correct subnuclear localization. Moreover, pUL51 was found in a complex with the terminase subunits pUL56 and pUL89. Our data provide evidence that pUL51 is crucial for HCMV genome cleavage-packaging and may represent a third component of the viral terminase complex. Interference with the interactions between the terminase subunits by antiviral drugs could be a strategy to disrupt the HCMV replication cycle.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Virology
Numberingvol. 87, no. 3, pp. 1720-1732
p-ISSN0022-538X
e-ISSN1098-5514
DOIhttps://doi.org/10.1128/JVI.01955-12
URN:NBNurn:nbn:hr:184:589071
Publication2013-02-01
Publication2012-11-21
Document URLhttp://jvi.asm.org/lookup/doi/10.1128/JVI.01955-12
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2019-02-25 22:56:17