Title Antimikrobni učinak gljiva
Title (english) Antimicrobial activity of mushrooms
Author Filip Brnobić
Mentor Maja Abram (mentor)
Committee member Darinka Vučković (predsjednik povjerenstva)
Committee member Ivana Gobin (član povjerenstva)
Committee member Marina Bubonja Šonje (član povjerenstva)
Granter University of Rijeka Faculty of Medicine (Department of Microbiology and Parasitology) Rijeka
Defense date and country 2019-07-12, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Medical Microbiology
Abstract Gljive su najrasprostranjeniji organizmi na Zemlji sa značajnom ulogom u odvijanju ekoloških procesa u brojnim, posebno šumskim, ekosustavima. Cilj ovog istraživanja je bio in vitro istražiti antimikrobnu aktivnost samoniklih gljiva Macrolepiota procera, Lactarius piperatus, Hydnum repandum, Clitocybe nebularis i Craterellus cornucopioides prikupljenih na području Primorsko-goranske županije.
Metanolni ekstrakti odabranih gljiva testirani su prema dvije gram-negativne (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) i dvije gram-pozitivne (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 292129) bakterije Antimikrobni učinak određen je korištenjem difuzijskih (disk-difuzija i difuzija u jažicama) i mikrodilucijske metode kojom su određene vrijednosti minimalne inhibitorne i minimalne baktericidne koncentracije.
U najvećoj ispitivanoj koncentraciji (200 mg/mL) sve su gljive pokazale inhibitorni učinak prema svim odabranim bakterijama, osim M. procera koja ni u najvećoj koncentraciji nije inhibirala rast bakterije E. faecalis. M. procera je pokazala naslabiju antimikrobnu aktivnost u usporedbi s ostalim ispitivanih gljivama. Ekstrakt gljive C. nebularis ima skromnu antimikrobnu djelotvornost, posebno prema gram-pozitivnim bakterijama. Najbolji antimikrobni učinak uočen je za gljive C. cornucopioides i H. repandum koje su u koncentraciji od 100 mg/mL djelovale bakteriostatski na sve odabrane bakterije. Od gram-pozitivnih bakterija osjetljivijim se pokazao S. aureus, a među gram-negativnim P. aeruginosa. Enterococcus faecalis bio je najrezistentniji među ispitivanim bakterijama Rezultati su pokazali da ekstrakti jestivih gljiva, posebno gljiva C. cornucopioides i H. repandum s iskazanim antimikrobnim učinkom, mogu biti izvor novih bioaktivnih spojeva s potencijalnom primjenom u medicini, farmaceutskoj i prehrambenoj industriji.
Abstract (english) Mushrooms are the most widespread organisms on Earth with a significant role in the ecological processes in numerous, especially forest, ecosystems. The aim of this study was to investigate in vitro the antimicrobial activity of Macrolepiotia procera, Lactarius piperatus, Hydnum repandum, Clitocybe nebularis and Craterellus cornucopioides collected in the forests of the Primorsko-goranska county.
Methanol extracts of the selected mushrooms were tested against two gram negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and two gram-positive bacteria (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 292129). The antimicrobial effect was determined using diffusion methods (disc diffusion and diffusion in the wells) and a microdilution method which determines minimal inhibitory and minimal bactericidal concentrations.
At the highest concentration tested (200 mg/mL), all fungi showed an inhibitory effect on all selected bacteria, except M. procera which did not inhibit the growth of bacterium E. faecalis evan at the highest concentration. M. procera showed the weakest antimicrobial activity compared to other investigated mushrooms. C. nebularis extract has modest antimicrobial efficacy, especially against gram-positive bacteria. The best antimicrobial effect was observed for fungi C. cornucopioides and H. repandum, which at concentrations of 100 mg/mL acted bacteriostatic against all selected bacteria. Among the gram-positive bacteria, S. aureus proved to be more susceptible to mushrooms extract, while among gram-negative more susceptible was P. aeruginosa. E. faecalis was the most resistant among the bacteria tested.
41
The results have shown that edible mushroom extracts, especially C. cornucopioides and H. repandum with expressed antimicrobial effects, can be the source of new bioactive compounds with potential use in medicine, as well as pharmaceutical and food industry.
Keywords
antimikrobna aktivnost
bakterije
ekstrakt gljive
Keywords (english)
antimicrobial activity
bacteria
mushroom extract
Language croatian
URN:NBN urn:nbn:hr:184:530295
Study programme Title: Medicine Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resource Text
File origin Born digital
Access conditions Open access
Terms of use
Created on 2020-01-23 09:34:22