Title PREŽIVLJAVANJE FRANCISELLA NOVICIDA U VODAMA IZ JEZERA LOKVE, LEPENICA I BAJER
Author Ivan Vidinić
Mentor Marina Šantić (mentor)
Committee member Darija Vukić Lušić (predsjednik povjerenstva)
Committee member Nada Starčević-Čizmarević (član povjerenstva)
Committee member Marina Šantić (član povjerenstva)
Granter University of Rijeka Faculty of Medicine (Department of Microbiology and Parasitology) Rijeka
Defense date and country 2019-09-12, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Medical Microbiology
Abstract F. tularensis je gram negativni kokobacil veličine 0,2-0,7 μm, asporogen, nepokretan i ne posjeduje pile. Vrsta Francisella tularensis obuhvaća četiri podvrste (tularensis, holarctica, mediasiatica i novicida) od kojih je Francisella tularensis subsp. tularensis (tip A) najvirulentnija i najpatogenija te uzrokuje tularemiju, bolest divljih životinja koja se može prenijeti na čovjeka. Ozbiljnost infekcije varira ovisno o mjestu ulaska bakterije, imunološkom stanju čovjeka i virulenciji uzročnika. Cilj ovog istraživanja bio je utvrditi sposobnost preživljavanja bakterije Francisella novicida u vodama iz jezera Gorskog kotra. Jezero Lokve, jezero Lepenica i jezero Bajer poslužili su kao izvor za uzimanje uzoraka voda za ispitivanje u praktičnom radu. Sva su tri jezera nastala umjetnim putem, odnosno izgradnjom brane koja zadržava vodu na odgovarajućem području u svrhu iskorištavanja energije vodotoka. U praktičnom radu je korišten bakterijski soj F. novicida U112, dok se za kultivaciju koristio BCYE agar. U radu su primijenjene i ostale hranjive podloge ovisno o bakteriji stalne mikroflore vode koja se proučavala. Uzorci su obrađeni metodom membranske filtracije. U vodama iz jezera Lokve, Lepenica i Bajer proučavale su se promjene broja bakterije F. novicida u sterilnim i nesterilnim uzorcima za svako jezero pojedinačno. Provedena je i usporedba svih uzoraka sterilnih voda iz sva tri jezera te svih uzoraka nesterilnih voda iz sva tri jezera. Također je praćena promjena broja bakterija stalne mikroflore u uzorcima voda navedenih jezera prije i nakon unosa F. novicida. Obradom rezultata uočeno je da F. novicida preživljava u ispitivanim vodama iz jezera Lokve, Lepenica i Bajer te da se bolje razmnožava u sterilnim, a lošije u nesterilnim uzorcima. Također, unosom F. novicida u uzorke s prirodnom mikroflorom dolazi do povećanja broja ostalih bakterija iz razloga što u „natjecanju“ za hranjive tvari uzimaju veći dio u odnosu na F. novicida pa joj time sprječavaju rast i razmnožavanje
Abstract (english) F. tularensis is a gram negative cocobacillus 0.2-0.7 μm in size, asporogenic, immobile and does not possess fimbriae. The species Francisella tularensis comprises four subspecies (tularensis, holarctica, mediasiatica and novicida) of which Francisella tularensis subsp. tularensis (type A) is the most virulent and most pathogenic. It is causative agent of tularemia, a wildlife disease that can be transmitted to humans. The severity of the infection varies depending on the site of entry of the bacterium, the human immune status and the virulence of the agent. The aim of this study was to determine the viability of Francisella novicida in the water from lakes of Gorski Kotar. Lake Lokve, Lake Lepenica and Lake Bajer served as sources for sampling water for practical work. All three lakes were created artificially, that is, by constructing a dam that holds water in the appropriate area for the purpose of harnessing the water flow. In the practical work, the bacterial strain F. novicida U112 was used, while BCYE agar was used for cultivation. Other nutrient media were also applied in the study, depending on the bacterium of the permanent microflora of the water under study. Samples were processed by membrane filtration. Changes in F. novicida number in sterile and non-sterile samples were studied in the waters of Lake Lokve, Lepenica and Bayer for each lake individually. A comparison of all sterile water samples from all three lakes and all non-sterile water samples from all three lakes was also performed. Changes in the number of bacteria in the permanent microflora in the water samples of these lakes before and after the introduction of F. novicida were also monitored. Based on the results obtained, it was observed that F. novicida survives in the tested waters of Lokve, Lepenica and Bajer lakes and that it reproduces better in sterile and worse in non-sterile samples. Also, the introduction of F. novicida into specimens with natural microflora results in an increase in the number of other bacteria due to the fact that in the "competition" for nutrients they take up a greater proportion than F. novicida and thus prevent its growth and reproduction.
Keywords
Francisella
tularemija
jezero Lokve
jezero Lepenica
jezero Bajer
Keywords (english)
Francisella
Tularemia
Lokve Lake
Lepenica Lake
Bayer Lake
Language croatian
URN:NBN urn:nbn:hr:184:849438
Study programme Title: Study of Sanitary Engineering Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resource Text
File origin Born digital
Access conditions Open access
Terms of use
Created on 2020-01-31 10:59:02