prikaz prve stranice dokumenta Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins
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Scientific paper - Original scientific paper
Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins
The Journal of Cell Biology, 195 (2011), 7; 1123-1140. https://doi.org/10.1083/jcb.201108121
Sideridou, Maria; Zakopoulou, Roubini; Evangelou, Konstantinos; Liontos, Michalis; Kotsinas, Athanassios; Rampakakis, Emmanouil; Gagos, Sarantis; Kahata, Kaoru; Grabušić, Kristina; Gkouskou, Kalliopi; Trougakos, Ioannis P.; Kolettas, Evangelos; Georgakilas, Alexandros G.; Volarević, Siniša; Eliopoulos, Aristides G.; Zannis-Hadjopoulos, Maria; Moustakas, Aristidis; Gorgoulis, Vassilis G. More authors...

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Sideridou, M., Zakopoulou, R., Evangelou, K., Liontos, M., Kotsinas, A., Rampakakis, E. ... Gorgoulis, V. G. (2011). Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins. The Journal of Cell Biology, 195. (7), 1123-1140. doi: 10.1083/jcb.201108121

Sideridou, Maria, et al. "Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins." The Journal of Cell Biology, vol. 195, no. 7, 2011, pp. 1123-1140. https://doi.org/10.1083/jcb.201108121

Sideridou, Maria, Roubini Zakopoulou, Konstantinos Evangelou, Michalis Liontos, Athanassios Kotsinas, Emmanouil Rampakakis, Sarantis Gagos, et al. "Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins." The Journal of Cell Biology 195, no. 7 (2011): 1123-1140. https://doi.org/10.1083/jcb.201108121

Sideridou, M., et al. (2011) 'Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins', The Journal of Cell Biology, 195(7), pp. 1123-1140. doi: 10.1083/jcb.201108121

Sideridou M, Zakopoulou R, Evangelou K, Liontos M, Kotsinas A, Rampakakis E, and sur.. Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins. The Journal of Cell Biology [Internet]. 2011 December 26 [cited 2024 November 26];195(7):1123-1140. doi: 10.1083/jcb.201108121

M. Sideridou, et al., "Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins", The Journal of Cell Biology, vol. 195, no. 7, pp. 1123-1140, December 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:684626. [Accessed: 26 November 2024]

Metadata
Title (english)Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins
AuthorMaria Sideridou
AuthorRoubini Zakopoulou
AuthorKonstantinos Evangelou
AuthorMichalis Liontos
AuthorAthanassios Kotsinas
AuthorEmmanouil Rampakakis
AuthorSarantis Gagos
AuthorKaoru Kahata
AuthorKristina Grabušić
AuthorKalliopi Gkouskou
AuthorIoannis P. Trougakos
AuthorEvangelos Kolettas
AuthorAlexandros G. Georgakilas
AuthorSiniša Volarević
AuthorAristides G. Eliopoulos
AuthorMaria Zannis-Hadjopoulos
AuthorAristidis Moustakas
AuthorVassilis G. Gorgoulis
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Molecular Medicine and Biotechnology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
E-cadherin (CDH1) loss occurs frequently in carcinogenesis, contributing to invasion and metastasis. We observed that mouse and human epithelial cell lines overexpressing the replication licensing factor Cdc6 underwent phenotypic changes with mesenchymal features and loss of E-cadherin. Analysis in various types of human cancer revealed a strong correlation between increased Cdc6 expression and reduced E-cadherin levels. Prompted by these findings, we discovered that Cdc6 repressed CDH1 transcription by binding to the E-boxes of its promoter, leading to dissociation of the chromosomal insulator CTCF, displacement of the histone variant H2A.Z, and promoter heterochromatinization. Mutational analysis identified the Walker B motif and C-terminal region of Cdc6 as essential for CDH1 transcriptional suppression. Strikingly, CTCF displacement resulted in activation of adjacent origins of replication. These data demonstrate that Cdc6 acts as a molecular switch at the E-cadherin locus, linking transcriptional repression to activation of replication, and provide a telling example of how replication licensing factors could usurp alternative programs to fulfill distinct cellular functions.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleThe Journal of Cell Biology
Numberingvol. 195, no. 7, pp. 1123-1140
p-ISSN0021-9525
e-ISSN1540-8140
DOIhttps://doi.org/10.1083/jcb.201108121
URN:NBNurn:nbn:hr:184:684626
Publication2011-12-26
ProjectNumber: 285948
Title (english): Enhancing University of Crete Medical School Scientific Excellence and Translational Research Potential in Human Diseases
Acronym: TRANSPOT
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 223151
Title (english): Understanding inflammation-associated tumorigenesis for the rational design of novel anti-cancer therapeutic strategies.
Acronym: INFLA-CARE
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 201630
Title (english): Genomic Instability in Cancer and Precancer
Acronym: GENICA
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 062-1081347-0370
Title (croatian): Odgovor stanica sisavaca na pogrešku u sintezi ribozoma in vivo
Leader: Siniša Volarević
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttps://rupress.org/jcb/article/195/7/1123/50560/Cdc6-expression-represses-Ecadherin-transcription
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) icon
Created on2020-02-03 18:11:38