prikaz prve stranice dokumenta The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium
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Scientific paper - Original scientific paper
The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium
American Journal of Reproductive Immunology, 81 (2019), 5; e13104. https://doi.org/10.1111/aji.13104
Mulac‐Jeričević, Biserka; Šućurović, Sandra; Gulić, Tamara; Szekeres‐Bartho, Julia

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Mulac‐Jeričević, B., Šućurović, S., Gulić, T. & Szekeres‐Bartho, J. (2019). The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium. American Journal of Reproductive Immunology, 81. (5). doi: 10.1111/aji.13104

Mulac‐Jeričević, Biserka, et al. "The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium." American Journal of Reproductive Immunology, vol. 81, no. 5, 2019. https://doi.org/10.1111/aji.13104

Mulac‐Jeričević, Biserka, Sandra Šućurović, Tamara Gulić and Julia Szekeres‐Bartho. "The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium." American Journal of Reproductive Immunology 81, no. 5 (2019). https://doi.org/10.1111/aji.13104

Mulac‐Jeričević, B., et al. (2019) 'The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium', American Journal of Reproductive Immunology, 81(5). doi: 10.1111/aji.13104

Mulac‐Jeričević B, Šućurović S, Gulić T, Szekeres‐Bartho J. The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium. American Journal of Reproductive Immunology [Internet]. 2019 March 22 [cited 2024 May 19];81(5). doi: 10.1111/aji.13104

B. Mulac‐Jeričević, S. Šućurović, T. Gulić and J. Szekeres‐Bartho, "The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium", American Journal of Reproductive Immunology, vol. 81, no. 5, March 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:677164. [Accessed: 19 May 2024]

Metadata
Title (english)The involvement of the progesterone receptor in PIBF and Gal‐1 expression in the mouse endometrium
AuthorBiserka Mulac‐Jeričević
AuthorSandra Šućurović
AuthorTamara Gulić
AuthorJulia Szekeres‐Bartho
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Biology and Medical Genetics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Problem: The progesterone-regulated genes, PIBF and Gal‐1, are key players in the feto-maternal immunological interaction. This study aims to investigate the expression of PIBF and Gal‐1 in WT and progesterone receptor KO models as well as subsequent effects of PIBF on decidualization of stromal cells. Method of the study: PRAKO, PRBKO and PRKO BALB/c mice were used for assessing the role of PR isoforms in PIBF induction. PIBF‐ and Gal‐1 mRNA expression in the uterus was tested by real‐time PCR. The effect of PIBF on decidualization of endometrial stromal cells was verified by anti-desmin immunofluorescence. Immunohistochemistry was used for testing PIBF expression in the uterus. Gal‐1, ERα and PR positive decidual NK cells were detected by immunofluorescence. Results: PIBF mRNA was significantly increased in progesterone‐treated WT mice, but not in PRKO and PRAKO mice. PIBF protein expression was reduced in the endometria of PRKO and PRAKO, but not in PRBKO mice. During a 6‐day culture, PIBF induced decidual transformation of endometrial stromal cells. PIBF expression in the mouse uterus was highest during the implantation window, while Gal‐1 mRNA expression continuously increased between day 2.5 and day 11.5 of gestation. Decidual NK cells express Gal‐1 and ERα, but not PR at day 7.5 murine pregnancy. Conclusion: PIBF produced via engagement of PRA, is highly expressed in the endometrium during the implantation window, and plays a role in decidualization. The concerted action of PIBF and Gal‐1 might contribute to the low cytotoxic activity of decidual NK cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleAmerican Journal of Reproductive Immunology
Numberingvol. 81, no. 5, e13104
p-ISSN1046-7408
e-ISSN1600-0897
DOIhttps://doi.org/10.1111/aji.13104
URN:NBNurn:nbn:hr:184:677164
Publication2019-03-22
Document URLhttps://onlinelibrary.wiley.com/doi/abs/10.1111/aji.13104
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-03-30 14:29:07