prikaz prve stranice dokumenta Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis
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Scientific paper - Original scientific paper
Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis
Neuroimmunomodulation, 20 (2013), 3; 152-163. https://doi.org/10.1159/000346546
Grubić Kezele, Tanja; Jakovac, Hrvoje; Tota, Marin; Čanadi Jurešić, Gordana; Barac-Latas, Vesna; Milin, Čedomila; Radošević-Stašić, Biserka

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Grubić Kezele, T., Jakovac, H., Tota, M., Čanadi Jurešić, G., Barac Latas, V., Milin, Č. & Radošević Stašić, B. (2013). Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis. Neuroimmunomodulation, 20. (3), 152-163. doi: 10.1159/000346546

Grubić Kezele, Tanja, et al. "Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis." Neuroimmunomodulation, vol. 20, no. 3, 2013, pp. 152-163. https://doi.org/10.1159/000346546

Grubić Kezele, Tanja, Hrvoje Jakovac, Marin Tota, Gordana Čanadi Jurešić, Vesna Barac Latas, Čedomila Milin and Biserka Radošević Stašić. "Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis." Neuroimmunomodulation 20, no. 3 (2013): 152-163. https://doi.org/10.1159/000346546

Grubić Kezele, T., et al. (2013) 'Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis', Neuroimmunomodulation, 20(3), pp. 152-163. doi: 10.1159/000346546

Grubić Kezele T, Jakovac H, Tota M, Čanadi Jurešić G, Barac Latas V, Milin Č, and sur.. Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis. Neuroimmunomodulation [Internet]. 2013 [cited 2024 May 02];20(3):152-163. doi: 10.1159/000346546

T. Grubić Kezele, et al., "Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis", Neuroimmunomodulation, vol. 20, no. 3, pp. 152-163, 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:893650. [Accessed: 02 May 2024]

Metadata
Title (english)Metallothioneins I/II Expression in Rat Strains with Genetically Different Susceptibility to Experimental Autoimmune Encephalomyelitis
AuthorTanja Grubić Kezele
AuthorHrvoje Jakovac
AuthorMarin Tota
AuthorGordana Čanadi Jurešić
AuthorVesna Barac-Latas
AuthorČedomila Milin
AuthorBiserka Radošević-Stašić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Compared to the Dark Agouti (DA), the Albino Oxford (AO) rat strain exhibits lower susceptibility to the induction of experimental autoimmune encephalomyelitis (EAE). Here, we investigated the potential contribution of the heavy metal-binding proteins metallothioneins (MTs) I/II to these effects. Rats were immunized with bovine brain homogenate emulsified in complete Freund's adjuvant or only with complete Freund's adjuvant. The expression patterns of MTs mRNA and proteins and tissue concentrations of Zn2+ and Cu2+ were estimated in the brain and in the liver on days 7 and 12 after immunization, by real-time PCR, immunohistochemistry and inductively coupled plasma spectrometry, respectively. Additionally, the hepatic transforming growth factor beta and nuclear factor kappa B immunoreactivities were tested. Clinical signs of EAE were not induced in AO rats, but they upregulated the expression of MT I/II proteins in the brain (hippocampus and cerebellum) and in the liver, similarly as DA rats. The transcriptional activation of MT-I occurred, however, only in DA rats, which accumulated also more zinc in the brain and in the liver. In contrast, intact AO rats had greater hepatic MT-I mRNA immunoreactivity and more Cu2+ in the hippocampus. Besides, in immunized AO rats a high upregulation of transforming growth factor beta and nuclear factor kappa B immunoreactivities was found in several hepatic structures (vascular endothelium, Kupffer cells and hepatocytes). Our data show that AO and DA rats differ in constitutive and inductive MT-I gene expression in the brain and in the liver, as well as in the hepatic cytokine profile, suggesting that these mechanisms may contribute to the discrepancy in the susceptibility to EAE.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleNeuroimmunomodulation
Numberingvol. 20, no. 3, pp. 152-163
p-ISSN1021-7401
e-ISSN1021-7401
DOIhttps://doi.org/10.1159/000346546
URN:NBNurn:nbn:hr:184:893650
Publication2013
ProjectNumber: 062-0621341-0061
Title (croatian): Masne kiseline i polifenoli u regeneraciji i reparaciji tkiva.
Leader: Čedomila Milin
Jurisdiction: Croatia
Funding stream: ZP
ProjectNumber: 062-0621341-1337
Title (croatian): Morfogenetska svojstva stresnih proteina gp96 i metalotioneina
Leader: Biserka Radošević-Stašić
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttp://bib.irb.hr/624329
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) icon
Created on2020-04-08 09:35:26