prikaz prve stranice dokumenta The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.
  Download
PDF 1.58 MB
Scientific paper - Original scientific paper
The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.
Viruses, 7 (2015), 8; 4582-601. https://doi.org/10.3390/v7082834
Steingruber, M; Socher, E; Hutterer, C; Webel, R; Bergbrede, T; Lenac, Tihana; Sticht, H; Marschall, M

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Steingruber, M., Socher, E., Hutterer, C., Webel, R., Bergbrede, T., Lenac, T. ... Marschall, M. (2015). The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.. Viruses, 7. (8), 4582-601. doi: 10.3390/v7082834

Steingruber, M, et al. "The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.." Viruses, vol. 7, no. 8, 2015, pp. 4582-601. https://doi.org/10.3390/v7082834

Steingruber, M, E Socher, C Hutterer, R Webel, T Bergbrede, Tihana Lenac, H Sticht and M Marschall. "The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.." Viruses 7, no. 8 (2015): 4582-601. https://doi.org/10.3390/v7082834

Steingruber, M., et al. (2015) 'The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.', Viruses, 7(8), pp. 4582-601. doi: 10.3390/v7082834

Steingruber M, Socher E, Hutterer C, Webel R, Bergbrede T, Lenac T, and sur.. The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.. Viruses [Internet]. 2015 [cited 2024 November 09];7(8):4582-601. doi: 10.3390/v7082834

M. Steingruber, et al., "The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.", Viruses, vol. 7, no. 8, pp. 4582-601, 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:583296. [Accessed: 09 November 2024]

Metadata
Title (english)The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.
AuthorM Steingruber
AuthorE Socher
AuthorC Hutterer
AuthorR Webel
AuthorT Bergbrede
AuthorTihana Lenac
AuthorH Sticht
AuthorM Marschall
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Replication of human cytomegalovirus (HCMV) is characterized by a tight virus-host cell interaction. Cyclin-dependent protein kinases (CDKs) are functionally integrated into viral gene expression and protein modification. The HCMV-encoded protein kinase pUL97 acts as a CDK ortholog showing structural and functional similarities. Recently, we reported an interaction between pUL97 kinase with a subset of host cyclins, in particular with cyclin T1. Here, we describe an interaction of pUL97 at an even higher affinity with cyclin B1. As a striking feature, the interaction between pUL97 and cyclin B1 proved to be strictly dependent on pUL97 activity, as interaction could be abrogated by treatment with pUL97 inhibitors or by inserting mutations into the conserved kinase domain or the nonconserved C-terminus of pUL97, both producing loss of activity. Thus, we postulate that the mechanism of pUL97-cyclin B1 interaction is determined by an active pUL97 kinase domain.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleViruses
Numberingvol. 7, no. 8, pp. 4582-601
p-ISSN1999-4915
DOIhttps://doi.org/10.3390/v7082834
URN:NBNurn:nbn:hr:184:583296
Publication2015
Document URLhttp://bib.irb.hr/821462
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-04-19 14:42:43