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master's thesis
SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA
Rijeka: University of Rijeka, Faculty of Medicine, 2020. urn:nbn:hr:184:150642
Vragović, Martina
University of Rijeka
Faculty of Medicine
Department of Chemistry and Biochemistry

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Vragović, M. (2020). SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:150642

Vragović, Martina. "SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA." Master's thesis, University of Rijeka, Faculty of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:184:150642

Vragović, Martina. "SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA." Master's thesis, University of Rijeka, Faculty of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:184:150642

Vragović, M. (2020). 'SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:184:150642

Vragović M. SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2020 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:184:150642

M. Vragović, "SINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:184:150642

Metadata
TitleSINTEZA I KARAKTERIZACIJA POLIMERNIH NANOČESTICA S KONTROLIRANIM OTPUŠTANJEM NITROKSIDNIH RADIKALA
Title (english)SYNTHESIS AND CHARACTERIZATION OF POLYMER NANOPARTICLES WITH CONTROLLED NITROXIDE RADICAL RELEASE
AuthorMartina Vragović
MentorDamir Klepac (mentor)
MentorSrećko Valić (komentor)
Committee memberSrećko Valić (predsjednik povjerenstva)
Committee memberMarin Tota (član povjerenstva)
Committee memberDamir Klepac (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Rijeka
Defense date and country2020-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Nitroksidni radikali su organske molekule koje imaju jedinstvena antioksidacijska svojstva te mogu suzbiti rast tumora. U in vivo uvjetima, ovi radikali ne mogu se učinkovito koristiti jer se nespecifično akumuliraju u zdravom tkivu te se brzo izlučuju iz organizma. U ovom radu istraživane su nanočestice temeljene na poli[N-(2-hidroksipropil)metakrilamidu] (poli(HPMA)) i kolesterolu koje sadrže TEMPONE radikal vezan hidrazonskom (RNP-H) i peptidnom vezom (RNP-P). Veličina i oblik nanočestica određeni su metodom krio-transmisijske elektronske mikroskopije. Utvrđeno je da su dobivene nanočestice sferičnog oblika prosječne veličine 25−50 nm. Otpuštanje radikala s nanočestica praćeno je metodom elektronske spinske rezonancije (ESR) u rasponu pH vrijednosti od 7,4 do 5,0. Pokazano je da u kiseloj sredini koja je karakteristična za tumorske stanice dolazi do brzog otpuštanja radikala vezanih hidrazonskom vezom dok nanočestice koje sadrže radikale vezane peptidnom vezom ostaju stabilne. Nasuprot tome, u fiziološkim uvjetima ne dolazi do značajnog oslobađanja radikala ni sa jedne vrste nanočestica. Ovim istraživanjem pokazano je da se polimerne nanočestice mogu koristiti kao nosači nitroksidnih radikala u novom pristupu terapiji tumora kada se žele izbjeći neželjene nuspojave slobodnih radikala i produljiti njihovo zadržavanje u organizmu.
Abstract (english)
Nitroxide radicals are organic molecules that possess unique antioxidant properties and can suppress tumor growth. Under in vivo conditions, these radicals can not be used effectively because they accumulate nonspecifically in healthy tissue and are rapidly removed from the body. In this work, we investigated nanoparticles based on poly[N-(2-hydroxypropyl)methacrylamide] (poly(HPMA)) and cholesterol containing a TEMPONE radical bound by a hydrazone (RNP-H) and peptide bond (RNP-P) respectively. The size and shape of the nanoparticles were determined by cryo-transmission electron microscopy. Spherical nanoparticles with an average size of 25–50 nm were obtained. The release of radicals from the nanoparticles was investigated by electron spin resonance (ESR) spectroscopy in the pH range from 7.4 to 5.0. It was shown that the radicals bound by hydrazone bond are rapidly released in an acidic environment which is characteristic for tumor cells, while the nanoparticles containing radicals bound by peptide bond remain stable. In contrast, there is no significant release of radicals from both types of nanoparticles under physiological conditions. This research has shown that polymer nanoparticles can be used as a carriers of nitroxide radicals in a new approach to tumor therapy when we want to avoid unwanted side effects of free radicals and prolong their retention in the body.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:150642
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-01-14 09:06:58