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Scientific paper - Original scientific paper
Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface
Frontiers in Cell and Developmental Biology, 8 (2020); 1-24. https://doi.org/10.3389/fcell.2020.563607
Lučin, Pero; Jug Vučko, Natalia; Karleuša, Ljerka; Mahmutefendić Lučin, Hana; Blagojević Zagorac, Gordana; Lisnić, Berislav; Pavišić, Valentino; Marcelić, Marina; Grabušić, Kristina; Brizić, Ilija; Lukanović Jurić, Silvija

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Lučin, P., Jug Vučko, N., Karleuša, Lj., Mahmutefendić Lučin, H., Blagojević Zagorac, G., Lisnić, B. ... Lukanović Jurić, S. (2020). Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface. Frontiers in Cell and Developmental Biology, 8., 1-24. doi: 10.3389/fcell.2020.563607

Lučin, Pero, et al. "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface." Frontiers in Cell and Developmental Biology, vol. 8, 2020, pp. 1-24. https://doi.org/10.3389/fcell.2020.563607

Lučin, Pero, Natalia Jug Vučko, Ljerka Karleuša, Hana Mahmutefendić Lučin, Gordana Blagojević Zagorac, Berislav Lisnić, Valentino Pavišić, et al. "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface." Frontiers in Cell and Developmental Biology 8 (2020): 1-24. https://doi.org/10.3389/fcell.2020.563607

Lučin, P., et al. (2020) 'Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface', Frontiers in Cell and Developmental Biology, 8, pp. 1-24. doi: 10.3389/fcell.2020.563607

Lučin P, Jug Vučko N, Karleuša Lj, Mahmutefendić Lučin H, Blagojević Zagorac G, Lisnić B, and sur.. Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface. Frontiers in Cell and Developmental Biology [Internet]. 2020 [cited 2024 November 26];8:1-24. doi: 10.3389/fcell.2020.563607

P. Lučin, et al., "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface", Frontiers in Cell and Developmental Biology, vol. 8, pp. 1-24, 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:376839. [Accessed: 26 November 2024]

Metadata
Title (english)Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface
AuthorPero Lučin
AuthorNatalia Jug Vučko
AuthorLjerka Karleuša
AuthorHana Mahmutefendić Lučin
AuthorGordana Blagojević Zagorac
AuthorBerislav Lisnić
AuthorValentino Pavišić
AuthorMarina Marcelić
AuthorKristina Grabušić
AuthorIlija Brizić
AuthorSilvija Lukanović Jurić
Author's institutionUniversity North
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Beta-herpesviruses develop a unique structure within the infected cell known as an assembly compartment (AC). This structure, as large as the nucleus, is composed of host-cell-derived membranous elements. The biogenesis of the AC and its contribution to the final stages of beta-herpesvirus assembly are still unclear. In this study, we performed a spatial and temporal analysis of the AC in cells infected with murine CMV (MCMV), a member of the beta-herpesvirus family, using a panel of markers that characterize membranous organelle system. Out of 64 markers that were analyzed, 52 were cytosolic proteins that are recruited to membranes as components of membrane-shaping regulatory cascades. The analysis demonstrates that MCMV infection extensively reorganizes interface between early endosomes (EE), endosomal recycling compartment (ERC), and the trans-Golgi network (TGN), resulting in expansion of various EE-ERC-TGN intermediates that fill the broad area of the inner AC. These intermediates are displayed as over-recruitment of host-cell factors that control membrane flow at the EE-ERC-TGN interface. Most of the reorganization is accomplished in the early (E) phase of infection, indicating that the AC biogenesis is controlled by MCMV early genes. Although it is known that CMV infection affects the expression of a large number of host-cell factors that control membranous system, analysis of the host-cell transcriptome and protein expression in the E phase of infection demonstrated no sufficiently significant alteration in expression levels of analyzed markers. Thus, our study demonstrates that MCMV-encoded early phase function targets recruitment cascades of host cell-factors that control membranous flow at the EE-ERC-TGN interface in order to initiate the development of the AC.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleFrontiers in Cell and Developmental Biology
Numberingvol. 8, pp. 1-24, 563607
e-ISSN2296-634X
DOIhttps://doi.org/10.3389/fcell.2020.563607
URN:NBNurn:nbn:hr:184:376839
Publication2020
ProjectNumber: IP-2014-09-9564
Title (croatian): Karakterizacija preuređivanja endosomalnih membrana tijekom rane faze infekcije mišjim citomegalovirusom koje vodi k formiranju kompleksa za stvaranje viriona
Title (english): Characterization of endosomal remodelling during the early phase of murine cytomegalovirus infection that leads to the development of the assembly complex
Acronym: AsCo
Leader: Pero Lučin
Jurisdiction: Croatia
Funding stream: IP
ProjectNumber: uniri-biomed-18-88
Title (croatian): Citomegalovirusni odjeljak za sklapanje viriona
Title (english): Cytomegalovirus assambly compartment
Leader: Pero Lučin
Jurisdiction: Croatia
Funding stream: Nema
Document URLhttps://www.frontiersin.org/article/10.3389/fcell.2020.563607/full
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-01-21 21:56:27