prikaz prve stranice dokumenta Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
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Scientific paper - Original scientific paper
Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
Bantao Journal, 18 (2020), 1; 24-30. urn:nbn:hr:184:001617
Katalinić, Nataša; Crnić Marčetić, Tajana; Mavrinac, Martina; Starčević, Alma; Balen, Anita; Balen, Sanja

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Katalinić, N., Crnić Marčetić, T., Mavrinac, M., Starčević, A., Balen, A. & Balen, S. (2020). Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen. Bantao Journal, 18. (1), 24-30. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, Nataša, et al. "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen." Bantao Journal, vol. 18, no. 1, 2020, pp. 24-30. https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, Nataša, Tajana Crnić Marčetić, Martina Mavrinac, Alma Starčević, Anita Balen and Sanja Balen. "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen." Bantao Journal 18, no. 1 (2020): 24-30. https://urn.nsk.hr/urn:nbn:hr:184:001617

Katalinić, N., et al. (2020) 'Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen', Bantao Journal, 18(1), pp. 24-30. Available at: https://urn.nsk.hr/urn:nbn:hr:184:001617 (Accessed 28 April 2024)

Katalinić N, Crnić Marčetić T, Mavrinac M, Starčević A, Balen A, Balen S. Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen. Bantao Journal [Internet]. 2020 [cited 2024 April 28];18(1):24-30. Available at: https://urn.nsk.hr/urn:nbn:hr:184:001617

N. Katalinić, T. Crnić Marčetić, M. Mavrinac, A. Starčević, A. Balen and S. Balen, "Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen", Bantao Journal, vol. 18, no. 1, pp. 24-30, 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:001617. [Accessed: 28 April 2024]

Metadata
Title (english)Assessment of Luminex Mean Fluorescence Intensity Values with Complement-Dependent Cytotoxicity Results in Detection of Antibodies Against Human Leucocyte Antigen
AuthorNataša Katalinić
AuthorTajana Crnić Marčetić
AuthorMartina Mavrinac
AuthorAlma Starčević
AuthorAnita Balen
AuthorSanja Balen
MBZ: 236875
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Clinical Laboratory Diagnostics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Introduction. Antibodies to human leukocyte antigens (HLA) are one of the major immune barriers to successful organ transplantation. In addition to complementdependent cytotoxicity (CDC) assay as a standard method for the HLA antibodies detection, more sensitive solid-phase assays like Luminex were introduced. The aim of this study was to define mean fluorescence intensity (MFI) cutoff values of HLA Class I antibodies detected by Luminex from those detected by CDC given the possibility to use only Luminex assay in HLA antibody screening. Methods. This is a retrospective analysis of the HLA antibody screening results of patients on a kidney transplant waiting list, performed at the Tissue Typing Laboratory, Clinical Hospital Center Rijeka, Croatia, from January 2012 to July 2019. The study included 1,665 sera tested in parallel by CDC and Luminex techniques. Results. Almost half sera contained HLA antibodies (47.9%), significantly more detected by the Luminex than a CDC method. Antibodies against HLA-A and HLA-B molecules had higher MFI values, relative to the HLA-C antibodies, as well as antibodies detected by Luminex and CDC than those detected by Luminex alone. A cutoff MFI≥9,204 for Luminex detected HLA Class I antibodies correlated with positivity in the CDC assay. Besides MFI, several factors need to be taken into consideration in interpreting test results to identify unacceptable antigen mismatch. Conclusion. The results of this analysis suggest that the current features of the Luminex technique provide the most benefit in the HLA antibody scre.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBantao Journal
Numberingvol. 18, no. 1, pp. 24-30
e-ISSN1312-2517
URN:NBNurn:nbn:hr:184:001617
Publication2020
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-03-03 19:25:38