prikaz prve stranice dokumenta Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies
  Download
PDF 698.71 KB
Scientific paper - Original scientific paper
Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies
Journal of Virological Methods, 293 (2021); 114173. https://doi.org/10.1016/j.jviromet.2021.114173
Bubonja-Šonje, Marina; Batičić, Lara; Abram, Maja; Cekinović Grbeša, Đurđica

Linked objects
COVID-19 | Virtual collection

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Bubonja Šonje, M., Batičić, L., Abram, M. & Cekinović Grbeša, Đ. (2021). Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies. Journal of Virological Methods, 293.. doi: 10.1016/j.jviromet.2021.114173

Bubonja Šonje, Marina, et al. "Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies." Journal of Virological Methods, vol. 293, 2021. https://doi.org/10.1016/j.jviromet.2021.114173

Bubonja Šonje, Marina, Lara Batičić, Maja Abram and Đurđica Cekinović Grbeša. "Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies." Journal of Virological Methods 293 (2021). https://doi.org/10.1016/j.jviromet.2021.114173

Bubonja Šonje, M., et al. (2021) 'Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies', Journal of Virological Methods, 293. doi: 10.1016/j.jviromet.2021.114173

Bubonja Šonje M, Batičić L, Abram M, Cekinović Grbeša Đ. Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies. Journal of Virological Methods [Internet]. 2021 April 27 [cited 2024 May 02];293. doi: 10.1016/j.jviromet.2021.114173

M. Bubonja Šonje, L. Batičić, M. Abram and Đ. Cekinović Grbeša, "Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies", Journal of Virological Methods, vol. 293, April 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:242267. [Accessed: 02 May 2024]

Metadata
Title (english)Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies
AuthorMarina Bubonja-Šonje
AuthorLara Batičić
AuthorMaja Abram
AuthorĐurđica Cekinović Grbeša
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Infectious Diseases)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Infectology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Immunology
Abstract (english)
Evidence is currently insufficient to know whether SARS-CoV-2 antibodies (Abs) protect from future infection and how long immunity will last. The kinetics of the immune response to SARS-CoV-2 infection and role of serology in estimating individual protective immunity is yet to be established. We evaluated diagnostic performances of three serological assays - Abbott Architect CMIA IgG, bioMerieux VIDAS ELFA IgG/IgM, and Diesse Chorus ELISA IgG/IgM, and analyzed longevity and potential neutralizing effect of SARS-CoV-2 Abs in COVID-19 patients. Clinical sensitivities of assessed IgG tests two to three weeks post symptom onset (PSO) were very high: 96.77 % for Architect, 96.77 % for Chorus, and 100.00 % for VIDAS. Sensitivities of two assessed IgM assays were moderate: 74.07 % for Chorus, and 76.92 % for VIDAS. Specificities were excellent for all assessed IgG assays: 99.01 % for Architect and 100 % for Chorus and VIDAS. Chorus and VIDAS IgM assays also achieved excellent specificity of 99.01 % and 100 %, respectively. In most cases IgG Abs were still present eight months PSO. Neutralizing antibodies were detected in majority of serum samples from convalescent patients. Serum samples from severe COVID-19 patients had higher antibody titers and higher neutralizing activity. We observed a strong positive correlation among SARS-CoV-2 IgG antibody titer and neutralizing activity. The strongest positive correlation to neutralizing activity was found for VIDAS IgG assay.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Virological Methods
Numberingvol. 293, 114173
p-ISSN0166-0934
DOIhttps://doi.org/10.1016/j.jviromet.2021.114173
URN:NBNurn:nbn:hr:184:242267
Publication2021-04-27
ProjectNumber: uniri-biomed-18-277
Title (croatian): Bakterijska patogeneza - od istraživanja do kliničke primjene
Leader: Maja Abram
Jurisdiction: Croatia
Funding stream: Sveučilište u Rijeci
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0166093421001129
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2021-05-13 19:57:48