Title Detekcija biofilma na keramičkim pločicama pomoću ATP bioluminiscencije Title (english) Biofilm detection on ceramic tiles using ATP bioluminescence Author Bruno Kovač Mentor Ivana Gobin (mentor)Committee member Dijana Tomić Linšak (predsjednik povjerenstva)Committee member Vanja Vasiljev (član povjerenstva)Committee member Ivana Gobin (član povjerenstva)Granter University of Rijeka Defense date and country 2021-09-10, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract ATP bioluminiscencija metoda je kojom se provodi mjerenje učinkovitosti higijenskih postupaka u raznim industrijskim pogonima, ustanovama zdravstvene skrbi, ali i u ugostiteljstvu. U prisutnosti ATP-a, luciferaza zajedno s kofaktorima D-luciferinom i kisikom producira svjetlo, odnosno energiju. Količina produciranog svjetla (energije) mjeri se luminometrom i izražava se u RLU (eng. Relative Light Units). Osim ATP-a iz mikroorganizama, mjeri se i ATP od ostataka proizvoda na površini. Ne postoji puno podataka o korištenju ove metode za detekciju biofilma te je stoga cilj rada bio prikazati učinkovitost sredstva za čišćenje Bis Duo Active na uništavanju bakterijskog biofilma na keramičkim pločicama in vitro. Na keramičkim pločicama dimenzija 2,5 x 2,5 cm stvoren je biofilm bakterija Staphylococcus aureus, Salmonella Typhimurium i Listeria monocytogenes tijekom 24 sata. Te su pločice zatim tretirane radnim koncentracijama sredstva za čišćenje tijekom 10 minuta. Nakon ispiranja mjerio se RLU pomoću luminometra te određivao CFU/mL nasađivanjem deseterostrukih razrjeđenja na Müller-Hinton agar i brojanjem poraslih kolonija. Također se ispitala korelacija dobivenih rezultata.
Obje radne koncentracije sredstva za čišćenje (5% i 20%) pokazale su se kao vrlo učinkovite u uništavanju stvorenog bakterijskog biofilma na keramičkim pločicama. Korišteno sredstvo za čišćenje pokazalo se najučinkovitije na primjeru redukcije broja bakterija na pločici biofilma S. aureus kod mjerenja vrijednosti RLU, dok je prilikom izračunavanja CFU/mL zaključeno da je najučinkovitije djelovalo na pločici biofilma L. monocytogenes. Gledajući postotke inhibicije, nešto slabiju djelotvornost sredstvo za čišćenje pokazalo je na primjeru pločice biofilma S. Typhimurium u oba slučaja. RLU i CFU/mL vrijednosti pokazuju vrlo jaku povezanost te koreliraju u vrijednosti 0,95770.
Zaključno, ATP bioluminiscencija pokazuje dobre rezultate kod detekcije biofilma na keramičkim pločicama za sve tri ispitivane bakterije te nakon tretmana sredstvom za čišćenje pokazuje jako dobru redukciju vrijednosti. Postotak inhibicije nakon primjene tretmana dezinficijensom kod RLU je najmanje 85,62%, dok je kod CFU/mL 98,30%.
Abstract (english) ATP bioluminescence is a method used to measure the effectiveness of hygienic procedures in various industrial plants, but also in catering. In the presence of ATP, luciferase together with the cofactors D-luciferin and oxygen produce light and energy, respectively. The amount of light (energy) produced is measured with a luminometer and expressed in RLU (Relative Light Units). In addition to ATP from microorganisms, ATP from product residues on the surface is also measured. There is not much data on the use of this method and, therefore, the aim of the research was to demonstrate the effectiveness of the cleaning agent Bis Duo Active on the destruction of bacterial biofilm on ceramic tiles in vitro. A biofilm of Staphylococcus aureus, Salmonella Typhimurium and Listeria monocytogenes was created on ceramic tiles with dimensions of 2.5 x 2.5 cm over 24 hours. These plates were then treated with working concentrations of detergent for 10 minutes. After washing, RLU was measured with a luminometer, and CFU/mL was determined by plating ten-fold dilutions on Müller-Hinton agar and counting the grown colonies. The correlation between the obtained results was also examined.
Both working concentrations of cleaning agent (5% and 20%) proved to be very effective on destroying the created bacterial biofilm on ceramic tiles. The cleaning agent used proved to be most effective in the example of reducing the number of bacteria on the S. aureus biofilm plate when measuring RLU values, while the CFU/mL calculation concluded that it had the most effective effect on the L. monocytogenes biofilm plate. Looking at the percentages of inhibition, the cleanser's effectiveness was slightly lower in the example of the S. Typhimurium biofilm plate in both cases. RLU and CFU/mL values show a very strong correlation and correlate in value 0,95770.
In conclusion, ATP bioluminescence shows good results in the detection of biofilm on ceramic tiles of all three tested bacteria and, after treatment with a cleaning agent, shows a very good reduction in value. The percentage of inhibition after disinfection treatment in RLU is at least 85,62%, while in CFU/mL 98,30%.
Keywords
ATP bioluminiscencija
biofilm
dezinficijens
Keywords (english)
ATP bioluminescence
biofilm
disinfectant
Language croatian URN:NBN urn:nbn:hr:184:210711 Study programme Title: Study of Sanitary Engineering Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2021-08-25 07:11:43
